氯丙嗪和异搏定对镉毒性的拮抗作用

Antagonistic effects of chlorpromazine and verapamil on toxicity induced by cadmium

目的探讨氯丙嗪(Chlorpromazine,CPZ)和异搏定(Verapamil,Ver)对镉毒性的影响。方法32只Wis-tar大鼠随机分成4组:对照组、单纯染镉组、氯丙嗪及异搏定干预组。测定肝和肾皮质镉、丙二醛(MDA)、谷胱甘肽(GSH)含量和谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性以及尿N-乙酰--βD氨基葡萄糖苷酶(NAG)和碱性磷酸酶(ALP)活性。结果单纯染镉组肝、肾皮质MDA、GSH含量和尿NAG和ALP活性显著高于对照组,而肾皮质SOD活性显著低于对照组。CPZ干预组肝和肾皮质MDA含量分别为单纯染镉组的75%,78%;肾皮质GSH含量显著低于单纯染镉组,而GSH-Px和SOD活性显著高于对照组和单纯染镉组。Ver干预组肝、肾皮质MDA和肾皮质GSH含量显著低于单纯染镉组,而肾皮质GSH-Px活性显著高于对照组,肾皮质SOD活性显著高于单纯染镉组。2个干预组尿NAG活性显著低于单纯染镉组。结论CPZ和Ver对镉毒性具有一定的拮抗作用。

Objective To study the effects of Chlorpromazine（CPZ） and Verapamil （Ver）on the toxicity induced by cadmium（Cd）. Methods Thirty-two Wistar rats were divided into four groups randomly, i. e., control group, Cd alone group, CPZ or Ver treatment group. Cd, Malondialdehyde（MDA）, Glutathione （GSH） contents and Glutathione peroxidatase （ GSH- Px）, Superoxide dismutase （ SOD ） activities in the liver and renal cortex were determined. Urinary N-acetyl-β-D-glucosaminidase（NAG） and alkaline phosphalase（ALP）activities were also measured. Results MDA, GSH contents in the liver and renal cortex and urinary NAG, ALP activities of Cd alone group were significantly higher than those of control group, but SOD activities in the renal cortex were significantly lower than those of control values. MDA contents in the liver and renal cortex of CPZ treatment group were lower than those of Cd alone group, but GSH-Px and SOD activities in the renal cortex were significantly higher than those of control and Cd alone groups. MDA contents in the liver and renal cortex and GSH contents in the renal cortex of Ver treatment group were significantly lower than those of Cd alone group, but GSH-Px activities in the renal cortex were significantly higher than control values, and SOD activities in the renal cortex were significantly higher than those of Cd alone group. Urinary NAG activities of CPZ and Ver treatment groups were significantly lower than those of Cd alone group. Conclusion CPZ and Ver had antagonistic effects on the toxicity induced by Cd.