红花(Carthamus tinctorius L.)种子醇溶蛋白遗传多样性分析

Genetic diversity of seeds prolamine in Carthamus tinctorius L.

利用A—PAGE（Acid—polyacrylamide gel electrophoresis）技术对来源于32个国家的53份红花材料进行了种子醇溶蛋白检测。结果表明，这些红花材料具有丰富的醇溶蛋白等位变异，共分离出15条迁移率不同的谱带。其中，每份材料可电泳出5-12条谱带，平均8．5条，所有材料有1条共有带。红花种子醇溶蛋白可作为评价红花遗传多样性的工具之一。不同材料的遗传相似系数（GS）变异范围为0．375—1．000，平均值为0．752。聚类分析结果表明，在GS值为0．752的水平上供试材料聚为6大类，其亲缘关系远近与地理来源关系不大。其中，来自中国的7份材料分别被聚在了3个大类中，表明中国红花种子醇溶蛋白遗传多样性比较丰富。

The genetic diversity of prolamine among 53 safflower accessions from 32 countries were investigated by A- PAGE. A total of 15 prolamine bands were separated by electrophoresis. Each accession has 5 to 12 prolamine bands with a mean of 8.5, and there is one common band in all accessions. The results suggested that there was high genetic variability in prolamine of Carthamus tinctorius. It was one of the method to evaluate the genetic diversity of safflower. The mean genetic similarity （GS） coefficient was 0. 752, ranging from 0. 375 to 1. 000. The cluster analysis showed that all accessions were clustered into 6 groups within the GS value of 0. 752. The genetic relationships of most accessions based on prolamine were not associated with their geographical distribution. Seven accessions from China were separated into 3 groups, which inferred big genetic diversity of seed prolamine of Carthmus tinctorius accessions from China.