芸芥自交亲和系与自交不亲和系SOD、POD和CAT酶活性

Studies on SOD、POD and CAT activity between self-compatible and self-incompatible lines in Eruca sativa Mill

对芸芥(Eruca sativaM ill)自交亲和系(SC)与自交不亲和系(SI)柱头SOD、POD和CAT三种酶活性进行了测定。自交亲和系和自交不亲和系在授粉后都能引起SOD、POD和CAT三种保护性酶活性的变化。比较SC和SI的酶活性发现,在未授粉时二者SOD酶活性差异显著,而POD和CAT酶活性则无显著性差异;异花授粉后SC和SI的三种酶活性表现了相似的趋势,在各个时间段上无差异;自花授粉后自交亲和系的酶活性显著高于自交不亲和系的酶活性。结果表明,SOD、POD和CAT三种酶活性的变化与自交亲和系所具有的自交亲和基因的调控有关。

SOD ,POD and CAT activities were measured between self- compatible line and self- incompatible lines. The results showed that the activity of SOD,POD and CAT increased after pollination in both lines. SOD activity was significantly different between self - compatible and self-incompatible lines before pollination, but POD and CAT activities were not different. SOD, POD and CAT activities of self- compatible and self- incompatible lines showed similar trend, and the enzyme activity was not significantly different at any stage between the lines after cross - pollination. The activity of self - compatible line was significant higher than self - incompatible line after pollination . The results showed that the change of enzyme activity correlated with the expression of self - compati- ble genes.