摘要
用固定化环糊精葡萄糖基转移酶催化淀粉环化反应，考察了反应时间、乙醇浓度和淀粉液化程度对环糊精产率的影响。环糊精产率最高达５４％，固定化酶经多次重复使用或长时间连续操作，酶活力降低较少，产品经纸色谱分离后用碘显色，发现产品主要为β－环糊精。
The immobilized cyclodextrin glycosyltransferase(CGTase) was used for preparation of cyclodextrin(CD).The influence of reaction time,concentration of alcohol and liquification degree of starch on yield of CD was discussed.The highest yield of CD could reach 54% and the main product was β-CD.The activity of immobilized CGTase lost very little after repeated use.
出处
《应用化学》
CSCD
北大核心
1996年第6期28-30,共3页
Chinese Journal of Applied Chemistry
关键词
固定化
葡萄糖基转移酶
环糊精
immobilization
cyclodextrin glycosyltransferase
cyclodextrin
