有机介质中辅酶NADH的电化学氧化

A Study on Electrochemical Oxidation of Reduced Nicotinamide Coenzyme NADH in Organic Media

用伏安法分别研究Ｎ，Ｎ′－二甲基甲酰胺和１，４－二氧六环介质中还原态烟酰胺辅酶ＮＡＤＨ在玻碳电极上的电化学氧化行为并测定某些动力学参数。ＮＡＤＨ的电氧化表现为完全不可逆过程，实验证明其过程伴有快速化学步骤。与在１，４－二氧六环介质中的相比，ＮＡＤＨ在Ｎ，Ｎ′－二甲基甲酰胺介质中的电氧化速度和扩散系数较小，但电流响应较为稳定。根据溶剂效应讨论了上述实验结果。

The behaviour of electrochemical oxidation of the reduced nicotinamide coenzyme NADH on glassy carbon was studied and some kinetic parameters were measured by means of voltammometry in the N-dimethylformamide(DMF)and 1,4-dioxane media respectively. The eletrochemical oxidation of NADH shows completely irriversable both in the two solvents,and the diagnostic experiments support that the process involves a fast chemical step.The oxidation rate and the diffusion coefficient of N ADH in DMP are smaller than those in 1,4-dioxane,but the response current is more stable in DMF. The experimental results are discussed by solvent effects.