猪腔前卵泡机械分离研究

Mechanical Isolation Methods of Porcine Preantral Follicles

本研究以分离到的卵泡数量、分离时间和卵泡在体外培养3 d后的存活率为指标比较了两种机械方法分离猪腔前卵泡的效果。结果表明:用针刮法分离猪腔前卵泡的数量(Φ<50μm:203600±59000;50μm≤Φ≤150μm:29.40±10.34;Φ>150μm:9.30±3.29)极显著(P<0.01)高于剪碎法(Φ<50μm:18900±12000;50μm≤Φ≤150μm:13.95±3.70;Φ>150μm:4.95±1.61),而且针刮法16.16±1.43 min分离时间比剪碎法(20.30±1.48)min短,(P<0.01)。体外培养3 d后,腔前卵泡存活率在两种方法之间没有明显差异。说明应用针刮法分离猪腔前卵泡能有效保护卵母细胞和颗粒细胞的正常形态以及基膜的完整性,从而使分离到的猪腔前卵泡维持正常的生理活性。

This study compared the effects of two mechanical isolation methods of porcine preantral follicles regarding the number, handling times of isolation preantral follicles and their livability after three days cuhureed in vitro as indexes. The results showed that needle scraping method harvested significantly more porcine preantral follicles （Φ〈50μ m：203600±59000, 50μm≤ Φ≤150μm： 29.40±10.34, Φ〉 150μm： 9.30±3.29 than cutting （Φ〈 50μ m： 18900±12000, 50μm≤Φ≤150μ m： 13.95±3.70, Φ〉150 μm： 4.95± 1.61）（P〈0.01）. The average handling time of needle scraping method for each ovary （16.16± 1.34） min was less than that of cutting （20.30±1.48） min （P〈0.01）. The livability of preantral follicles after three days in vitro culture was not significant different between the two groups. The results indicated needle scraping mechanical isolation could be used to maintain normal physiological functions of preantral follicles because it kept the natural figures of oocytes and granulosa cells and preserved the integrity of basement membrane.