摘要
基于在碱性介质中，己烯雌酚对N-溴代丁二酰亚胺-鲁米诺化学发光体系的阻抑作用，建立了测定己烯雌酚的流动注射化学发光分析新方法，探讨并优化了流动注射化学发光的分析条件。该方法测定己烯雌酚的线性范围为1．5×10^-8～2．6×10^-7g／mL，检出限为5．0×10^-9g／mL，对1．0×10^-7g／mL的己烯雌酚标准溶液进行11次测定，相对标准偏差为2．6％。并应用于饲料、己烯雌酚片剂中的己烯雌酚的测定。
In alkaline medium, a novel flow injection chemiluminescent method has been developed for the determination of diethylstilbestrol, based on the inhibition of diethylstilbestrol on the chemiluminescence reaction between luminol and N-bromosuccinimide. Conditions of this chemiluminescent reaction have been discussed and optimized. The linear range of determination is 1.5×10^-8～ 2.6×10^-7 g/mL for diethylstilbestrol, the detection limit is 5.0×10^-9 g/mL, and the relative standard deviation （RSD） （n = 11 ） is 2.6% for 1.0× 10^-7 g/mL diethylstilbestrol. The method has been applied to determine the content of diethylstilbestrol in feed and medicine with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第8期10-12,共3页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金资助(20277005)
