西洋参冠瘿组织悬浮培养及其人参总皂苷的含量测定

Study on Supension Culture and Quantitative Determination of Total Ginsenosides from the Crown Gall of Panax quinquefolium

目的:在西洋参冠瘿组织固体培养的基础上,进一步考察其悬浮培养条件及人参总皂苷含量,为利用西洋参冠瘿组织大规模生产药用成分提供科学根据。方法:考察固体培养和悬浮培养对西洋参冠瘿组织生长和人参总皂苷累积的影响,并检测悬浮培养液中碳源的消耗。结果:冠瘿组织在悬浮培养中生长速度快,生长量和总皂苷产量分别达到0.776 g DW/flask和19.4 mg/flask,是固体培养的2.11和1.64倍;培养基中蔗糖利用率达到91.8%,远远高于西洋参愈伤组织悬浮培养中的蔗糖利用率。结论:西洋参冠瘿组织悬浮培养既可大大提高生物工程产品的生物量,又能获得较高含量的人参总皂苷,可为大规模生产药用活性物质提供参考。

The influences of different cultural node on the crown gall culture of Panax quinquefolium and its ginsenosides contents were studied. The faster growth rate was obtained in suspension culture. And the dry weight（DW） of crown gall and the accumulation of total ginsenoside content reached 0. 776 g DW/flask and 19. 4 mg/flask, which were 111% and 64% higher than solid culture, respectively. The results also indicated that the utilization （by percentage） of sucrose was 91.8%. The investigation provided useful information for following researches on fermentation culture and large-scale production of ginsenosides.