摘要
目的:建立评价香加皮质量优劣的指纹图谱分析方法。方法:以香加皮药材为研究对象,应用RP-HPLC法,色谱柱为Krom asil C18(250 mm×4.6 mm,5μm),乙腈-0.025%磷酸水二元梯度洗脱模式,流速为1.0 m l/m in,检测波长为220 nm,建立了15批不同产地香加皮样品的RP-HPLC色谱指纹图谱。结果:对各产地药材指纹图谱进行聚类分析,根据聚类分析相似度分析结果,将香加皮药材分为4类,选定其中6批优质样品建立共有模式,并应用“中药色谱指纹图谱相似度评价系统2004版”对各产地药材进行了质量评价。结论:该方法简便、可靠,为有效控制香加皮药材质量提供依据。
Objective : To establish the RP-HPLC fingerprint analysis for the quality control of Cortex Periplocae. Methods : HPLC fingerprint and analysis method of Cortex Periplocae were studied. Kromasil C18 column(250 mm×4.6 mm,5μm) was used, with mixture of acetonitrile and 0.025% phosphate solution as mobile phase in a gradient mode. The flow rate was 1.0 ml/min. The wavelength of measurement was 220 nm. 15 Batches of Cortex Periplocae were determined. Results: The 15 samples were classified as 4 clusters by cluster analysis and the 6 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by“ Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004”. Conclusion: The method is simple and reliable. It can be used to evaluate and control the quality of Cortex Periplocae conveniently.
出处
《中药材》
CAS
CSCD
北大核心
2006年第7期715-717,共3页
Journal of Chinese Medicinal Materials
关键词
香加皮
RP-HPLC
指纹图谱
Cortex Periplocae
RP-HPLC
Chromatographic fingerprint