HPLC测定附子及其炮制品中3种双酯型生物碱的含量

Determination of three kind of diester diterpenoid alkaloids(DDAs) in Aconitum carmichaeli and its processed products by HPLC

目的：建立同时测定附子中3种双酯型生物碱——新乌头碱、乌头碱、次乌头碱含量的HPLC分析方法。方法：Zorbax Eclipse XDB C18柱，40mmol乙酸铵缓冲液-乙腈，梯度洗脱，流速1.0mL·min^-1，检测波长230nm。结果：3种双酯型生物碱新乌头碱、乌头碱、次乌头碱均达到了较好的分离，分别在0．0357-1.784μg（r=0．9999），0.0126-0．632μg（r=0．9997）和0．0334-1．672μg（r=0．9997）呈良好的线形关系。结论：该方法简便、快速、灵敏，可有效地用于附子的鉴别和内在质量控制。

Objective： To establish an HPLC method for the determination of three kind of diester diterpenoid alkaloids （DDAs） in Aconitum carmichaeli and its processed products namely mesaconitine, aconitine and hypaconitine. Method： A Zorbax Eclipse XDB C18 scolumn was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL· min^-1 and a detection wavelength of 230 nm. Result. Mesaconitine, aconitine and hypaconitine, were separated, and the calibration curves of them were in good linearity over the range of 0.035 7-1.784 μg （ r = 0.999 9）, 0.012 6-0.632μg （ r=0.999 7） and 0.033 4-1.672μg （ r=0.999 7） respectively. Conclusion： This method is simple and accurate. It can be used in the identification and quality control of A.carmichaeli.