摘要
目的:研究四物汤对环磷酰胺(CTX)损伤致血虚证小鼠骨髓蛋白质组的影响,旨在分子水平上探讨四物汤促进骨髓造血的作用机制。方法:采用CTX 250 mg.kg-1腹腔注射制成化学损伤型血虚证小鼠模型,利用蛋白质组学技术研究四物汤治疗后的差异蛋白质组。结果:研究发现CTX使小鼠骨髓中12个蛋白表达上调、3个蛋白表达下调,四物汤可使CTX造模后这些表达改变的蛋白有不同程度恢复。结论:四物汤可能通过影响与细胞凋亡、造血干祖细胞增殖分化有关的蛋白质而促进骨髓造血。
Objective: To study the effects of Siwu decoction on protein expression of blood deficiency mice induced by cyclophosphamide(CTX) and discuss the possible molecular mechanism on blood enriching function of Siwu decoction. Method: Blood deficiency mice were established by injecting ip with 250 mg·kg-^1 CTX. Protcomic technologics were applied to identify the different protein. Result: Siwu decoction could restore the changes of 12 up-regulated and 3 down-regulated proteins in bone marrow of blood deficiency mice induced by cyclospbosphamide. Conclusion: Siwu decoction could effect expression of proteins which functions including apoptosis, proliferation and differentiation of the haematopoietic stem/progenitor cell. The regulation in the molecular level might be the mechanism of stimulating hematopoiesis in bone marrow fo siwu decocetion.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第14期1172-1175,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(30371725)
关键词
四物汤
CTX
骨髓
蛋白质组学
Siwu decoction
cyclosphosphamide
bone marmw
proteomics