四物汤对环磷酰胺致血虚证小鼠骨髓蛋白质组的影响

Effects of Siwu decoction on bone marrow protein expression of blood deficiency mice induced by cyclophosphamide

目的:研究四物汤对环磷酰胺(CTX)损伤致血虚证小鼠骨髓蛋白质组的影响,旨在分子水平上探讨四物汤促进骨髓造血的作用机制。方法:采用CTX 250 mg.kg-1腹腔注射制成化学损伤型血虚证小鼠模型,利用蛋白质组学技术研究四物汤治疗后的差异蛋白质组。结果:研究发现CTX使小鼠骨髓中12个蛋白表达上调、3个蛋白表达下调,四物汤可使CTX造模后这些表达改变的蛋白有不同程度恢复。结论:四物汤可能通过影响与细胞凋亡、造血干祖细胞增殖分化有关的蛋白质而促进骨髓造血。

Objective： To study the effects of Siwu decoction on protein expression of blood deficiency mice induced by cyclophosphamide（CTX） and discuss the possible molecular mechanism on blood enriching function of Siwu decoction. Method： Blood deficiency mice were established by injecting ip with 250 mg·kg-^1 CTX. Protcomic technologics were applied to identify the different protein. Result： Siwu decoction could restore the changes of 12 up-regulated and 3 down-regulated proteins in bone marrow of blood deficiency mice induced by cyclospbosphamide. Conclusion： Siwu decoction could effect expression of proteins which functions including apoptosis, proliferation and differentiation of the haematopoietic stem/progenitor cell. The regulation in the molecular level might be the mechanism of stimulating hematopoiesis in bone marrow fo siwu decocetion.