栀子提取物抗病毒试验研究

Antivirus effects of extract from gardenia

目的:观察栀子提取物ZG对小鼠流感病毒性肺炎模型的影响和体外对多种病毒致细胞病变作用的影响。方法:小鼠经滴鼻感染流感病毒造成肺炎模型,观察肺部炎症、死亡率和生命延长率,体外抗病毒试验采用CPE法,按Reed-Muench方法计算TCO,TC5O,IC5O,TI。结果:栀子提取物ZG对流感病毒致小鼠肺部炎症有明显抑制作用,可明显降低流感病毒感染后小鼠的死亡率、延长存活时间,同时降低小鼠血清中NO含量;在体外对流感病毒等6种病毒引起的细胞病变有明显抑制作用。结论:栀子提取物ZG对流感病毒等6种病毒有明显抑制作用,其对小鼠流感病毒性肺炎的抑制作用与降低血清中NO含量有关。

Objective： To observe the effect of the extract from gardenia on influenza viral pneumonia in mice and virus-induced cytopathic effect. Method： The mice were infected by influenza virus in nasal, the lung inflammation, mortality rate and life elongation rate were observed respectively. The anti-viral activity of the extract from gardenia was accessed by cytopathic effect （CPE） in vitro and 0% toxicity concentrafion （TC0）, 50% toxicity concentration （TC50）, 50% inhibitor concentration （IC50）, therapeutic index （TI） were determined by ReedMuench method. Result：The pneumonia induced by influenza virus in mice was inhibited significantly by the extract from gardenia, as the mortality rate decreased and the life elongation rate increased remarkably. Meanwhile the NO content in serum decreased significantly; The cytopathic effect induced by six kinds of viruses was inhibited remarkably. Conclusion： The six kinds of viruses were inhibited significantly by the extract from gardenia which inhibitory effect on mice influenza viral pneumonia was related to the NO content decreased.