苏郁胶囊对抑郁模型小鼠行为及海马神经元损伤的影响

Effect of Suyu capsule on behavior and injury of hippocampal neurons in depression model mice

目的:探究苏郁胶囊对慢性应激抑郁模型小鼠行为、海马神经元损伤及海马突触体内游离Ca2+浓度的影响。方法:将60只昆明种雄性小鼠分为正常组、模型组、苏郁胶囊高、中、低剂量组(22.8,11.4,5.7 g.kg-1)。采用长期不可预见性中等强度应激造成小鼠抑郁模型。测定各组小鼠体重变化,Open-field法和糖水消耗实验测定各组小鼠的行为变化;Nissl染色法观察海马CA1,CA3区神经元形态及锥体细胞数目;以Fura-2负载及荧光分光光度计检测海马突触体内游离Ca2+浓度。结果:与正常组比较,模型组小鼠体重增长缓慢(P<0.01),水平活动得分和垂直活动得分显著减少(P<0.01),糖水消耗量明显降低(P<0.01);海马CA3区锥体细胞数量明显减少(P<0.01);海马突触体内游离Ca2+浓度明显升高(P<0.01);苏郁胶囊高剂量组于第14,21天时小鼠体重增长数明显高于模型组(P<0.05);苏郁胶囊高剂量能增加抑郁小鼠的水平活动得分(P<0.05);苏郁胶囊高、中剂量组能增加抑郁小鼠的垂直活动得分(P<0.01,P<0.05);苏郁胶囊高、中、低剂量能明显增加抑郁小鼠的糖水消耗量(P<0.01,P<0.05,P<0.05);苏郁胶囊高、中、低剂量组能明显增加海马CA3区锥体细胞数量(P<0.01,P<0.05,P<0.05);苏郁胶囊高、中、低剂量组能明显降低抑郁小鼠海马突触体内游离Ca2+浓度(P<0.01,P<0.01,P<0.05)。结论:苏郁胶囊能改善小鼠的抑郁样行为;保护抑郁模型小鼠海马神经元损伤,其可能与苏郁胶囊抑制海马神经细胞外Ca2+内流,阻止Ca2+超载相关。

Objective： To observe the effect of Suyu capsule on behavior, injury of hippecampal neurons and Ca^2＋ ion in hippocampal synaptic in the depression model mice. Method： Sixty male Kunming mice were randomly divided into 5 groups, the control group, the model group and three Suyu capsule groups （ the doses were 22.8, 11.4, 5.7g·kg^-1 respectively）. The model was established by separation and chronic unpredictable mild stimulation. The increased weight and crossing score, rearing score were measured by open-field and sweet water consumption of mice. Cone cell and configuration of neuron in CA1, CA3 region of hippocampus were observed by Nissl. The concentration of hippocampal synaptic Ca^2＋ ion was detected by fluorirnetry. Result： Comparing with the mice of control, the increased weight was slowered （P〈0.01 ）, the scores of rearing and crossing were decreased （P〈0.01 ）, sweet water consumption were decreased too （P〈0.01 ）, numbers of cone cell in CA3 region of hippocampus were decreased obviously （P〈0.01）, and Ca^2＋ ion in hippocampal synaptic was increased obviously. Comparing with the mice of model, Suyu capsule（22.8 g·kg^-1）could increase the increased weight on the 14th and 21 st day obviously （P〈O.05） ; Suyu capsule （22.8 g·kg^-1） could increase the scores of crossing obviously （P〈O.05）, Suyu capsule （22.8,11.4 g·kg^-1 ） could increase the scores of rearing obviously（ P〈O.01, P〈O.05） ; Suyu capsule （22.8, 11.4, 5.8 g·kg^- 1 ） could increase sweet water consumption obviously （ P〈O.01, P 〈O.05, P〈O.05 ; Suyu capsule （22.8, 11.4, 5.8 g· kg^-1） could increase numbers of cone cell in CA3 region of hippocampus obviously（ P 〈 0.01, P 〈 0.05, P 〈 0.05） ; Suyu capsule（22.8, 11.4, 5.8 g·kg^-1）decreased Ca^2＋ ion in hip0ocampal synaptic with dose-effect relationship（P〈0.01, P〈0.01, P〈O.05）. Conclusion： Suyu capsule can improve all the symptoms of the depression model mice and protect injury of hippocampal neurons in the depression model mice. The possible mechanism of action is to restrict Ca^2＋ ion overfreight.