IO~－_3－I~－_－结晶紫－阿拉伯胶体系分光光度法测定生物样品中的碘

Spectrophotometric Determination of Iodine in Biological Samples with IO - 3 I - Crystal Violet Acacia gum System

在硫酸介质中，在过量碘化钾及阿拉伯胶存在下，ＩＯ－３氧化Ｉ－生成Ｉ－３，Ｉ－３再与结晶紫形成离子缔合物，缔合物的最大吸收波长为５５２ｎｍ，表观摩尔吸光系数ε５５２＝５．３８×１０５Ｌ·ｍｏｌ－１·ｃｍ－１，ＩＯ－３量在０～１２μｇ／２５ｍＬ范围内服从比耳定律．用于生物样品中碘的测定。

A new spectrophotometric method for the determination of iodine has been developed. Based on the IO - 3 oxidation, the I - forms I - 3, then I - 3 reacts with crystal violet(CV) to form an ion associatc of . The maximum absorbance is at 552nm. The method has very high sensitivity due to a high molar absorptivity of 5.38×10 5L·mol -1 ·cm -1 . Beers law is obeyed in the range of 0￣12μg IO - 3/25mL. The method has been applied to the determination of iodine in biological samples, with satisfactory results.