免疫重建荷人卵巢癌-SCID鼠模型的建立

Establishment of Hu-PBL-SCID Mice Model of Ovarian Carcinoma

[目的]探讨建立具有人免疫学特征的卵巢癌-SCID鼠模型的可行性。[方法]将16只雌性SCID小鼠随机分为4组。A组:磷酸盐缓冲液(PBS)0.5ml;B组为荷HO-8910组:HO-8910细胞1×106个/只;C组为人免疫重建组:PBL细胞4×107个/只,进行免疫重建;D组为荷HO-8910免疫重建组:PBL细胞4×107个/只,24h后腹腔注射HO-8910细胞1×106个/只,观察各组小鼠的生物学及免疫学特性。[结果]免疫重建鼠的骨髓、肝脏、脾脏、胸腺、淋巴结、外周血、腹腔液中检测到人源性细胞;荷HO-8910细胞免疫重建组小鼠移植瘤体积小于荷HO-8910组小鼠;各组均无移植物抗宿主病(GVHD)发生。[结论]初步建立免疫重建荷人卵巢癌-SCID鼠模型,为卵巢癌治疗的临床前研究提供了理想动物模型。

[Purpose]To investigate the feasibility of establish a human ovarian carcinoma model in human lymphocyte-engrafted to severe combined immunodeficient （hu-PBL-SCID） mice. [Methods]Sixteen SCID mice were randomly divided into 4 groups： group A with intraperitoneally phosphate buffered saling （0.5ml each mouse）, group B with human ovarian carcinoma cells HO-8910（1×10^6 each mouse）, group C with human peripheral blood lymphocyte （PBL） （4×10^7 each mouse） for immune reconstruction, and group D with PBL （4×10^7 each mouse） and HO-8970 （1×10^6 each mouse） cells. The biological and immunological features of each groups were evaluated. [Results]Human immunological cells were detected in bone marrow, liver, spleen, thymus, lymphoid mode, peripheral blood and peritoneal fluid of hu-PBL-SCID mice. The volume of tumor were less in the reconstruction of human immunsystem group. No graft-versus-host disease （GVHD） was found in all groups. [Conclusion]A human ovarian carcinoma model has been established in hu-PBL-SCID mice which is an ideal animal model for preclinical research and treatment for ovarian carcinoma.