摘要
应用聚合酶链反应(PCR)检测了153例各型乙型肝炎患者的血清HBVDNA,并与ELISA法检测的血清HBeAg和抗HBe结果比较.结果HBeAg阳性89例,PCR法HBVDNA检出率为92.13%;抗HBe阳性27例,PCR法HBVDNA检出率为51.85%;e系统用性37例,PCR法HBVDNA检出率为40.54%.提示抗HBe血清学转换不能作为乙型肝炎病毒复制与非复制、病变活动与静止的唯一指标,而应该用或同时用HBVDNA作为指标才可靠.
We examined the serum HBV DNA in 153 patients with various types of hepatitis B by using PCR,and compared the results of serum HBeAg and antiHBe detected by ELISA. The positive rate of HBV DNA detected by PCR was 92. 13% in 89 patients with positive HBeAg detected by means of ELISA,and it was 51.85% in 27 patients with positive anti-HBe. The positive rate of HBV DNA detected by PCR was 40. 54% in 37 patients with negative e antigen antibodies system. It is suggested that the transition of positive HBeAg to positive anti-HBe couldn't be the only standard marker whether HBV duplicated or not,or the disease was inactive form or not. It'S more reliable to examine HBV DNA alone or combination with it at the sametime.
出处
《科技通报》
1996年第4期243-245,共3页
Bulletin of Science and Technology