聚合酶链反应检测乙型肝炎患者血清HBVDNA与ELISA检测HBeAg、抗HBe比较研究

Comparing Research between Serum HBVDNA Detected by PCR and HBeAg, AntiHBe Detected by ELISA

应用聚合酶链反应（ＰＣＲ）检测了１５３例各型乙型肝炎患者的血清ＨＢＶＤＮＡ，并与ＥＬＩＳＡ法检测的血清ＨＢｅＡｇ和抗ＨＢｅ结果比较．结果ＨＢｅＡｇ阳性８９例，ＰＣＲ法ＨＢＶＤＮＡ检出率为９２．１３％；抗ＨＢｅ阳性２７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为５１．８５％；ｅ系统用性３７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为４０．５４％．提示抗ＨＢｅ血清学转换不能作为乙型肝炎病毒复制与非复制、病变活动与静止的唯一指标，而应该用或同时用ＨＢＶＤＮＡ作为指标才可靠．

We examined the serum HBV DNA in 153 patients with various types of hepatitis B by using PCR,and compared the results of serum HBeAg and antiHBe detected by ELISA. The positive rate of HBV DNA detected by PCR was 92. 13% in 89 patients with positive HBeAg detected by means of ELISA,and it was 51.85% in 27 patients with positive anti-HBe. The positive rate of HBV DNA detected by PCR was 40. 54% in 37 patients with negative e antigen antibodies system. It is suggested that the transition of positive HBeAg to positive anti-HBe couldn't be the only standard marker whether HBV duplicated or not,or the disease was inactive form or not. It'S more reliable to examine HBV DNA alone or combination with it at the sametime.