摘要
目的:建立人食管癌Eca109细胞系膜蛋白分离和初步纯化方法。方法:使用改良的Neville法提取细胞膜,在pH6.3的条件下,用去垢剂TritonX100和辛基β葡糖苷把细胞膜上的蛋白溶解下来,以超滤法纯化膜蛋白并分组。结果:在pH6.3的条件下,适当的去垢剂与膜蛋白质量之比使用去垢剂辛基β葡糖苷时为61;使用TritonX100时为21。超滤法将膜蛋白分为相对分子质量<3000、3000~10000、<10000、10000~30000、30000~100000、>100000共6个组分。结论:获得适当的去垢剂与膜蛋白质量比,并获得了不同的膜蛋白组分,为进一步研究人食管癌Eca109细胞肿瘤抗原奠定了基础。
Aim : To establish the initial methods to isolate and purify the membrane proteins of human esophageal carcinoma Eca-109 cell line. Methods:The cell membrane was extracted with the improved Neville's method( pH 6.3). The membrane proteins were dissolved out from cytomembrane with the detergents Triton X-100 or Octyl-β-D-glucopyranoside, respectively. Results: The appropriate ratio of detergent (Octyl-β-D-glucopyranoside) to protein was 6 : 1. In the Triton X-100 group the ratio was 2 : 1. The membrane proteins were allocated into 6 components including Mr 〈 3 000, 3 000 N 10 000, 〈 10 000, 10 000 N30 000, 30 000 - 100 000, 〉 100 000. Conclusion: This study provides foundation to investigate the tumor antigen of human esophageal carcinoma Eca-109 cell line.
出处
《郑州大学学报(医学版)》
CAS
北大核心
2006年第4期607-610,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关基金资助项目324410035
关键词
食管癌
细胞株
膜蛋白
esophageal carcinoma
cell line
membrane protein