苦参素对人胃癌细胞系MGC-803增殖的抑制作用

Inhibitive effect of oxymatrine on the human carcinoma cell line MGC-803

目的:观察苦参素对人胃癌细胞系MGC803的增殖抑制作用。方法:人胃癌细胞系MGC803接种培养24h后,实验组分别加入不同浓度苦参素(0.25g/L、0.5g/L、1g/L、2g/L、4g/L),阳性对照组与阴性对照组分别加入氟尿嘧啶(2g/L)和培养液,继续培养12h,24h,36h,48h,采用MTT法测定苦参素对MGC803细胞的增殖抑制情况。判定苦参素的剂量与MGC803细胞增殖活性间的关系,计算细胞抑制率和群体倍增时间。结果:苦参素在0.25～4g/L范围内,对MGC803细胞的增殖活性具有抑制作用,与阴性对照组比较,差异有统计学意义(P<0.001),且该作用随剂量增加而增加。与阴性对照组比较,阳性对照组和实验组细胞群体倍增时间增加。结论:苦参素对人胃癌细胞系MGC803有明显的增殖抑制作用,且具有时间、剂量效应关系。

Aim： To investigate the inhibition effect of oxymatrine （OM） on the human gastric carcinoma cell line MGC-803. Methods： MGC-803 cells were cultured for 24 h and allocated into different experimental groups. OM groups were added with 0.25 g/L, 0.5 g/L, 1 g/L,2 g/L, and 4 g/L OM, and the positive control group and negative control group were added with 2 g/L 5-fluorouracil and culture fluid, respectively. After being cultured for 12 h, 24 h, 36 h, 48 h, the proliferation inhibition of MGC-803 cells was assessed by MTT assay. The relation between the dose and cell proliferation viability was analyzed. Cell inhibitory rate and the doubling time were evaluated. Results： In the range of 0.25 -4 g/L, OM had obvious inhibitive effects on the proliferation of MGC-803 cells in a dose-dependent manner compared with the negative control group （P 〈 0.00l ）. Compared with negative control group, the doubling time of both positive control group and experimental groups was prolonged. Conclusion： OM has significant inhibition effects on the proliferation of MGC-803 cells in a time-dependent and dose-dependent way.