人脑星形细胞瘤组织中转化生长因子α与肿瘤增殖细胞核抗原检测

Detection of transforming growth factor alpha and tumor proliferating cell nuclear antigen in human astrocytoma tissue

中图分类号R739.41摘要目的:探讨人脑星形细胞瘤组织中转化生长因子α(TGFα)蛋白的表达与肿瘤增殖细胞核抗原(PCNA)及预后的关系。方法:采用免疫组化SP法检测50例人脑星形细胞瘤(其中星形细胞瘤18例,间变性星形细胞瘤20例,多形性胶质母细胞瘤12例)石蜡标本和10例正常脑组织中TGFα蛋白表达和PCNA标记指数(PCNALI),并随访比较50例人脑星形细胞瘤患者术后1a的生存率。结果:10例正常脑组织中TGFα、PCNALI蛋白无表达;50例人脑星形细胞瘤组织中TGFα蛋白表达总阳性率为60.0%(30/50),总PCNALI为(67.86±28.68)%;随着肿瘤病理分级的增高,TGFα蛋白表达和PCNALI也显著增高,各级别组间差异有统计学意义(P<0.05);TGFα蛋白表达和PCNALI呈正相关(r=0.864,P<0.01),TGFα蛋白表达强弱和PCNALI高低反映患者预后(P<0.01)。结论:人脑星形细胞瘤中存在TGFα和PCNA蛋白高表达,联合检测TGFα蛋白和PCNALI对评估人脑星形细胞瘤的生物学行为具有重要意义。

Aim ： To study the expression of transforming growth factor α（TGF-α） and proliferating cell nuclear antigen（PCNA） in human astrocytoma tissue. Methods：The expression of TGF-α and PCNA protein among 50 surgical specimens of human astrocytoma and 10 cases of normal brain tissue was detected using immunohistochemical staining method. All patients were followed up. Results： The total positive rates of TGF-α protein was 60.0% （30/50） , and the total PC- NA-LI was 67.86% ±28.68% in 50 surgical specimens of human astrocytoma. The expression of TGF-α and PCNA-LI increased with the increase of pathological grade （ P 〈 0. 05 ）. There was positive correlation between TGF-α and PCNA-LI （r =0. 864 ,P 〈0. 01 ）. The expression of TGF-α and PCNA protein was related to the patients＇prognosis （P 〈 0.01 ）. Conclusion ： There are overexpressions of TGF-α and PCNA protein in human astrocytoma tissue. Combined examination of TGF-α and PCNA protein has significance in evaluating the biological behavior of human astrocytoma.