表皮生长因子及红霉素对小鼠生殖道沙眼衣原体感染的抑制作用

Prevention effects of EGF and erythromycin on genital track infection of chlamydia trachomatis in mice

目的:通过衣原体感染动物模型的建立及不同因素干预,探讨并比较表皮生长因子(EGF)及红霉素对生殖道沙眼衣原体(CT)感染的影响,探索新的抑制沙眼衣原体感染的方法和思路。方法:用经过筛查的8～12周清洁级小鼠72只,制成沙眼衣原体生殖道感染模型,分别用EGF及红霉素干预,统计并比较不同时间每组小鼠阴道感染率及阳性感染者阴道排菌量情况。结果:由对照组第8dCT阳性感染率为79.2%可知沙眼衣原体动物感染模型制作成功;在感染第8d、第18dEGF干预组感染率分别为45.8%、33.3%,红霉素干预组为33.3%、4.2%,与对照组的79.2%、75.0%相比差异有统计学意义,且阳性感染者中阴道脱落菌量明显少于对照组。结论:EGF及抗生素通过不同的抗菌机制降低小鼠生殖道CT感染的发生率并能降低衣原体的毒力,从而为预防衣原体感染找到新的策略打下了实验基础。

Aim ： To explore the preventive effects of EGF and erythromycin on genital track infection of chlamydia trachomatis （CT） in mice. Methods： Seventy-two 8 - 12 week-old experimental mice were allocated into three groups： group A ： model group, group B： EGF group, and group C ： erythromycin group. All the 3 groups were infected with CT, group B was treated with EGF and group C, with erythromycin. The chlamydia shedding and the rate of infection were studied. Resuits： Compared with group A, groups B and C had relatively lower CT infection rate and CT cervicovaginal shedding. Conclusion： Both EGF and erythromycin can reduce the CT infection and cervicovaginal chlamydia shedding by different mechanism, which provides a new way to prevent CT infection.