子宫内膜异位症患者腹腔液及血清中白细胞介素12和肿瘤坏死因子-α检测

Detection of interleukin-12 and tumor necrosis factor-α in peritoneal fluid and serum of patients with endometriosis

目的:探讨子宫内膜异位症(内异症)患者腹腔液及血清中白细胞介素12(IL12)和肿瘤坏死因子α(TNFα)的变化及意义。方法:采用酶联免疫吸附双抗体夹心法(ELISA)检测26例内异症患者(Ⅰ、Ⅱ期11例,Ⅲ、Ⅳ期15例)及16例对照组(其他妇科手术,术中可见腹腔积液者)腹腔液及血清中IL12和TNFα含量。结果:内异症组腹腔液及血清IL12含量均低于对照组,2组腹腔液IL12均低于同组的血清含量,差异有统计学意义(P<0.01)。内异症组Ⅲ、Ⅳ期腹腔液IL12((19.56±0.83)ng·L-1)和血清IL12((23.62±0.59)ng·L-1)均低于Ⅰ、Ⅱ期腹腔液((23.59±0.56)ng·L-1)和血清((28.71±0.54)ng·L-1)含量,差异均有统计学意义(P均<0.01);内异症组腹腔液及血清TNFα含量均高于对照组,2组腹腔液TNFα含量均高于同组的血清含量,差异有统计学意义(P<0.01)。内异症组Ⅲ、Ⅳ期腹腔液TNFα((60.24±0.96)ng·L-1)和血清TNFα((49.90±1.36)ng·L-1)均高于Ⅰ、Ⅱ期腹腔液((53.37±0.89)ng·L-1)和血清((42.20±0.99)ng·L-1)含量,差异均有统计学意义(P均<0.01)。结论:IL12和TNFα在内异症发病中起重要作用,检测其含量变化有利于该病的诊断和治疗。

Aim： To study the changes of interleukin-12 （IL-12） and tumor necrosis factor-α（TNF-α）in peritoneal fluid and serum of patients with endometriosis. Methods：IL-12 and TNF-α levels in peritoneal fluid and serum of 26 patients with endometriosis and 16 control cases were detected by enzyme linked immunosorbent assay （ELISA）. ReSultS： IL-12 levels in peritoneal fluid and serum of endometriosis group were lower than those in control group. IL-12 levels of both groups in peritoneal fluid were lower than those in serum （P 〈0.01 ）. IL-12 in peritoneal fluid （ （19.56± 0.83 ）ng·L^-1 ） and IL-12 in serum （ （23.62 ±0.59）ng ·L^-1） of stage Ⅲ,Ⅳ in endometriosis group were lower than IL-12 in peritoneal fluid （ （ 23.59 ± 0.56） ng · L^-1 ） and in serum （ （ 28.71 ± 0.54） ng· L^ -1 ） of stage Ⅰ , Ⅱ （ both P 〈 0.01 ）. TNF-α levels in peritoneal fluid and serum of endometriosis group were higher than those in control group. TNF-α levels of both groups in peritoneal fluid were higher than those in serum （P 〈 0.01 ）. TNF-α in peritoneal fluid （ （60.24 ± 0.96） ng · L^-1 ） and TNF-α in serum （ （49.90 ± 1.36）ng · L^-1 ） of stage 111 ,IV in endometriosis group were higher than TNF-α in peritoneal fluid （（53.37 ± 0.89）ng· L^-1） and TNF-α in serum （（42.20 ± 0.99）ng· L^-1） of stage Ⅰ ,Ⅱ （both P 〈 0.01 ）. Conclusion： IL-12 and TNF-α play an important role in the pathogenesis of endometriosis. The detection of IL-12 and TNF-α will be helpful in the diagnosis and treatment of this disease.