宫颈鳞癌组织中上皮性钙黏附蛋白、CD_(44)V_6、nm23H_1的表达

Expression of E-cadherin, CD_ (44)V_6 and nm23-H_1 in cervical squamous cell carcinoma tissue

目的:探讨子宫颈鳞癌组织中上皮性钙黏附蛋白(Ecadherin,Ecad)、CD44V6和nm23H1的表达。方法:应用免疫组织化学技术,以14例正常宫颈上皮为对照,分别检测94例子宫颈浸润性鳞癌(Ⅰ～Ⅱ期59例,Ⅲ～Ⅳ期35例,高分化(Ⅰ级)23例,中等分化(Ⅱ级)46例,低分化(Ⅲ级)25例)及其中46例淋巴结转移组织中Ecad、CD44V6和nm23H1的蛋白表达。结果:正常宫颈、宫颈癌组织及淋巴结转移组织中Ecad蛋白、CD44V6、nm23H1蛋白阳性表达差异均有统计学意义(P<0.05)。Ecad,CD44V6的表达均与组织学分级、临床分期及淋巴结转移有关,nm23H1仅与临床分期和淋巴结转移相关(P均<0.05)。子宫颈鳞癌组织中,Ecad表达与CD44V6表达呈负相关(r=-0.225,P<0.05),CD44V6表达与nm23H1表达呈负相关(r=-0.282,P<0.05)。结论:Ecad、CD44V6和nm23H1可能直接参与子宫颈鳞癌的浸润、转移过程。

Aim ： To study the expression of E-cadherin （E-cad） , CD44 V6 and nm23-H1 in cervical squamous cell carcinoma tissue （CSCC）. Methods： The expression of the E-cad, CD44 V6 and nm23-H1 was detected in 14 cases of normal cervical mucusa, 94 cases of invasive CSCC and 46 cases of cervical carcinoma with lymph node metastases using immunohistochemistry. Results： The expression of E-cad, CD44V6, and nm23-H1 in 3 groups had significant difference （P 〈 0.05 ）. The expression of E-cad, CD44 V6 was correlated with histological grades, clinical stage and lymph node metastasis, while that of nm23-H1 was correlated with clinical stage and lymph node metastasis （P 〈 0.05 ）. The expression of E-cad was negatively correlated With CD44V6 （ r = - 0. 225 ,P 〈 0.05 ） , and the expression of CD44 V6 was negatively correlated with nm23-H1 （ r = - 0. 282, P 〈 0.05 ）. Conclusion ： E-cad, CD44 V6 and nm23-H1 may involve in the process of the invasion and metastasis in CSCC.