广东汉族人十二指肠溃疡和HLA-DQB1等位基因的关联研究

A study on HLA-DQB1 allele associated with genetic susceptibility to duodenal ulcer in Guangdong Hans

目的探讨广东地区汉族人人类白细胞抗原-DQB1(humanleukocyteantigen-DQB1,HLA-DQB1)等位基因与十二指肠溃疡(duodenalulcer,DU)的遗传关联。方法应用聚合酶链反应-序列特异性引物核苷酸分型技术,对105例DU患者、105名正常人的HLA-DQB1等位基因进行分析。结果DU患者HLA-DQB1*0602等位基因频率与正常对照组比较明显增高,差异有统计学意义(P=0.001,RR=4.533,Pc=0.014)。结论HLA-DQB1*0602与十二指肠溃疡有一定关联,HLA-DQB1等位基因分布在十二指肠溃疡患者与正常人之间存在着差异。

Objective To study the genetic susceptibility of HLA-DQB1 alleles to duodenal ulcer in Chinese Hans from Guangdong area around. Methods Hundred and five patients with duodenal ulcer and hundred and five healthy controls were examined for HLA-DQB1 genotypes. HLA-DQB1 allele typing was carried out by polymerase chain reaction with sequence specific primers （PCR-SSP）. Results The allele frequency of HLA-DQB1 ＊ 0602 in patients with duodenal ulcer （64.8%） was significantly higher than that in healthy controls （14.3%）. Conclusion These findings suggest that HLA-DQB1 ＊ 0602 is a susceptible gene to duodenal ulcer in Guangdong Hans of China. And at HLA- DQB1 site, there are immunogenetic differences between duodenal ulcer patients and healthy controls.