一株产脂肪酶芽孢杆菌的发酵条件及酶学特性

One Strain of Bacillas sp Producing Lipase and Its Enzymatic Properties

通过选择性培养基初筛获得脂肪酶活性较高的一株芽孢杆菌,利用摇瓶发酵优化发酵条件,结果表明,菌株产酶的最适条件为:在37℃条件下,接种量为1 mL,250 mL摇瓶的装液量为30 mL,培养时间48 h,转速175 r.min-1。发酵培养基添加的最佳碳源是1.5%麸皮,最佳氮源是2.5%的酪素。对酶特性做了初步分析,酶作用的最佳pH值7.5,最佳温度42℃。

One strain of bacillus, sp producing lipase was screened by selecting media from feculence, which come from meat process factory. The optimal fermentation conditions in shaking flask was studied. At temperature of 37℃, 1mL inoculum, volume 30mL in 250mL flask, time 48h, and rotate speed 175rpm, the producing lipase ability of this bacteria reached highest. The best of fermentation media was additive carbon source 1.5% bran and nitrogen source 2.5% casein. The lipase properties of this strain were also studied. The optimum temperature was 42℃,while the optimum pH was 7.5.