白细胞介素-6在2型糖尿病患者腹部皮下和网膜脂肪组织中的表达及其与胰岛素抵抗的关系

Expression of IL-6 in subcutaneous and omental adipose tissue of type 2 diabetes and their correlation with insulin resistance

目的探讨白细胞介素-6(IL-6)基因在2型糖尿病(T2DM)患者腹部皮下和网膜脂肪组织中的表达情况,及其与胰岛素抵抗的关系。方法用半定量RT-PCR方法检测22例正常人(对照组)和20例T2DM(糖尿病组)患者的大网膜与皮下脂肪组织IL-6 mRNA的表达水平,并测量体重、血压、腰围、臀围、血糖、空腹胰岛素、血脂和胰岛素敏感指数(IAI)。结果①糖尿病组网膜和皮下脂肪的IL-6 mRNA表达量均高于对照组(P<0.05);对照组网膜组织的IL-6 mRNA表达量高于皮下脂肪组织(P<0.05),而糖尿病组网膜和皮下脂肪组织差异无显著性(P>0.05)。②将两组数据合并,网膜和皮下脂肪的IL-6 mRNA表达量与腰臀比、胰岛素抵抗指数(HOMA-IR)呈正相关(P<0.05),与血浆IAI呈负相关(P<0.05),与其他因素均无相关性(均P>0.05)。③多元逐步回归分析发现,皮下脂肪IL-6 mRNA表达量与HOMA-IR、腰臀比相关(P<0.05),网膜IL-6 mRNA表达量与HOMA-IR相关(P<0.05)。结论T2DM患者网膜和皮下脂肪组织IL-6 mRNA表达水平升高,可以作为胰岛素抵抗的一个重要参数。

Objective To study the expression of interleukin-6 gene mRNA in subcutaneous and omental adipose tissue of type 2 diabetes mellitus paheat （ T2DM）. Methods Semi-quantitative RT-PCR was used to detect the expression of interleukin-6 gene mRNA in omental and abdominal subcutaneous adipose tissue of 20 cases with T2DM and 22 case of non-diabetes controls. Results （ 1 ） The interleukin-6 mRNA expression in subcutaneous and omemal adipose tissue was significantly increased in patients with T2DM than that of non-diabetes controls （ P 〈 0.01 ）. Compared with subcutaneous adipose tissue,the interleukin-6 mRNA expression in omental adipose tissue was significantly increased in non-diabetes controls （ P 〈 0.01 ）, but that was not in patients with T2DM （ P 〉 0.05 ）. （ 2 ） The expression level of IL-6 mRNA in all omental and abdominal subcutaneous adipose tissue were positively correlated with waist/hip ratio （ WHR ） and HOMA-IR （ P 〈 0.05 ）, but was negatively with plasma IAI （ P 〈 0.05. （ 3 ） Muhi-stepwise regression analysis demonstrated that the expression level of IL-6 mRNA in abdominal subcutaneous adipose tissue was significantly correlated with HOMA-IR and WHR（ P 〈 0.05 ） , and that the expression level of IL-6 mRNA in omental adipose tissue were correlated with HOMA-IR （ P 〈 0.05 ）. Conclusion IL-6 mRNA expres- sions in subcutaneous and omental adipose tissue are significantly higher in cases with T2DM, which seems to be an important parameter of insulin resistance.