对氧磷酶2基因311Cys/Ser多态性与2型糖尿病合并大血管病变的相关性

The Association of Paraoxonase 2 Gene 311 Cys/Ser Polymorphism with Macrovascular Disease in Type 2 Diabetes Mellitus Patients

目的探讨对氧磷酶2基因311 Cys/Ser多态性与山东青岛地区2型糖尿病患者合并大血管病变的关系。方法通过抽提基因组DNA并应用聚合酶链反应扩增包含对氧磷酶2基因311位点的基因片段,然后应用聚合酶链反应限制片长多态性技术检测对氧磷酶2基因311 Cys/Ser多态性在2型糖尿病合并大血管病变组、单纯2型糖尿病组以及正常对照组的基因频率。结果山东青岛地区人群存在对氧磷酶2基因311 Cys/Ser多态性。2型糖尿病合并大血管病变组对氧磷酶2基因的3种基因型(CC、CS、SS)的构成比与单纯2型糖尿病组和正常对照组比较差异具有显著性(P<0.05或P<0.01),S等位基因频率较单纯2型糖尿病组和正常对照组显著增高(P<0.05或P<0.01);携带S等位基因的个体患糖尿病大血管病变的风险为非携带者的2.932倍;对氧磷酶2基因311 Cys/Ser多态性不同基因型亚组间血脂水平无明显差异。结论在山东青岛地区人群中,对氧磷酶2基因311 Cys/Ser多态性与2型糖尿病合并大血管病变具有相关性,其S等位基因可能是该地区2型糖尿病合并大血管病变的危险因素之一。

Aim To investigate the association between taraoxonase 2 gene 311 Cys/Ser polymorphism and macrovascular disease in type 2 diabetes mellims patients. Methods A ease-control study of 272 subjects （including randomly selected 183 type 2 diabetes mellitus patients with or without macrovascular disease and 89 healthy controls） was performed. Genomic DNA was extracted from the subjects＇ peripheral blood leukocytes. The paraoxonase 2 gene 311 Cys/Ser polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism analysis with Ddel Ⅰ digestion. Results Paraoxonase 2 gene 311 Cys/Ser polymorphism was detected in population of Qingdao. The genetype distribution （ CC, CS and SS） of paraoxonase 2 gene 311 Cys/Ser polymorphism showed significant differences between type 2 diabetes meiitus complicated with macrovaseular disease group and the other two groups （type 2 diabetes mellitus group ＆ healthy group）, the former had significantly higher S allele frequenoy （P〈0.05 or P〈0.01）. The ratio of diabetic macrovascular disease, if S allele existed , increased 2.932 times. Levels of serum lipids in different genotypes of paraoxonase 2 gene 311 Cys/Ser in cases showed no evident differenees. Conclusions The paraoxonase 2 gene 311 Cys/Ser polymorphism is associated with type 2 diabetes mellitus complicated with macrovascular disease in Qingdao＇ s population. S allele may be a risk factor for type 2 diabetes mellitus complicated with macrovascular disease.