实验性弥散性血管内凝血大鼠淋巴循环的变化

Changes in lymph circulation in rats with experimental disseminated intravascular coagulation

目的探讨实验性弥散性血管内凝血(DIC)大鼠淋巴循环的变化。方法32只雄性Wistar大鼠按随机数字表法分为DIC组(n=16)和假手术组(n=16),每组中8只动物用于肠系膜淋巴微循环观察,另8只进行淋巴动力学检测。颈静脉注射高分子右旋糖酐(dextran500)制备Wistar大鼠实验性DIC模型,通过淋巴学方法,观察DIC时大鼠淋巴循环的变化。结果DIC时,肠系膜微淋巴管(ML)收缩性、肠淋巴流量、淋巴细胞输出量明显降低,淋巴液中有少量单核细胞,并且淋巴液黏度较高。经生理盐水治疗后,ML收缩性、肠淋巴流量、淋巴细胞输出量均显著升高,淋巴液中有大量单核细胞出现,与假手术组比较差异均有显著性(P均<0.05),且淋巴液黏度明显低于DIC组(P<0.05)。结论Wistar大鼠实验性DIC时,淋巴循环障碍表现为ML收缩性降低、淋巴循环转运功能障碍和淋巴液黏度增高。

Objective To explore changes in lymph circulation in rats with experimental disseminated intravascular coagulation （DIC）. Methods Thirty-two male Wistar rats were randomly divided into two groups： DIC group and sham-operation group, with 16 rats in each group. In each group, 8 rats were used to observe the lymphatic circulation of intestinal mesentery, and the others were used to observe lymphatic dynamics. Experimental DIC model was replicated by injecting dextran 500 via jugular vein in Wistar rats. The changes in lymph circulation were observed in these rats with lymphatology methods. Results During the phase of DIC, the contractility of mesenteric micro-lymphatic （ML）, the intestinal lymph flux, lymphocytes output were obviously decreased, and there was a few monocytes in the lymph fluid, but the lymph fluid viscosity was increased. After treatment with normal saline （NS）, the contractility of ML, the intestinal lymph flux, lymphocytes output were obviously increased, and there was a large amount of monocytes in the lymph fluid, with significant difference compared with sham-operation group （all P〈0.05）. Moreover, the lymph fluid viscosity was decreased compared with sham-operation group （P〈0. 05）. Conclusion The disturbance in lymph circulation mainly includes depression of contractility of lymphatics, the transport function of lymph circulation, and increased lymph fluid viscosity.