米诺环素对大鼠和小鼠脑外伤的部分保护作用

Incomplete protective effects of minocycline on traumatic brain injury in rats and mice

目的:研究半合成四环素类药物米诺环素对中枢神经损伤的保护作用。方法:在大鼠开放性脑外伤、小鼠闭合性脑外伤和脑冷冻伤3种脑损伤模型,于手术前2 d开始,每天2次预先给予米诺环素45 m g/kg ip;手术前30 m in及手术后1 h,各给药1次;手术后第1、2天,每天给药1次。观察手术后行为学变化,14 d后计算脑损伤面积及神经元数量。结果:米诺环素对大鼠开放性脑撞击伤,除了加快肢体功能(爬板角度)恢复外,对其他指标无明显作用;对小鼠脑闭合伤,能抑制神经元减少,但对行为学变化无明显作用;对小鼠脑冷冻伤,能减少死亡率及损伤面积,对神经元减少的作用不明显。结论:米诺环素对不同动物的不同脑外伤有部分保护作用。

Objective： To evaluate protective effect of minocycline,a semisynthetic tetracycline derivative on different traumatic brain injuries in rats and mice. Methods： The opened brain trauma was induced in rats and the closed head injury and cold brain injury were induced in mice. In 3 brain trauma models,minocycline （45 mg/kg,ip） was administered twice daily for 2 d before the operation,at 30 min before and 1 h after the operation,and once daily for 2 d following the operation （totally 8 doses in 5 d）. After the operation,the behavioral alteration was observed daily,lesion area and survival neuron density were measured at the end of the experiments （14 d after the injuries）. Results： For rat opened traumatic injury,minocycline promoted the recovery of hindlimb motor activity （inclined board angle）,but did not alter other indexes. For mouse closed head traumatic injury,minocycline reduced the neuron loss,but did not improve behavioral dysfunction. For mouse cold injury-induced trauma, minocycline reduced death rate and lesion area,but did not remarkably improve behavior and neuron loss. Conclusion： Minocycline only has an incomplete neuroprotective effect on different brain traumatic injuries in rats and mice.