黄芪注射液穴位注射治疗慢性盆腔炎的实验研究

Experimental treatment of chronic pelvic inflammatory disease in rats with acupoint injection of astragalus parenteral solution

目的:观察黄芪注射液穴位注射对慢性盆腔炎大鼠模型的治疗作用。方法:将50只W is-tar大鼠随机分成6组,除正常组和假手术组外,其余造成慢性盆腔炎模型。黄芪注射液穴注组和生理盐水穴注组选用关元、足三里进行穴位注射;药物对照组用妇科千金片灌胃。观察各组大鼠子宫的组织形态学变化,检测血清IL-2、TNF-α含量。结果:模型组病理形态学检查显示为慢性炎症改变,黄芪穴注组和妇科千金片组改变不明显;模型组TNF-α含量明显高于正常组和黄芪穴注组,血清IL-2含量显著低于其它5组。黄芪针穴注组IL-2、TNF-α含量接近正常。结论:黄芪针穴注治疗慢性盆腔炎大鼠具有较好的疗效,与模型组相比有显著性差异,并且优于生理盐水穴注组及妇科千金片组。

Objective： To observe the effect of acupoint injection with astragalus parenteral solution in rat chronic pelvic inflammatory disease （CPID）. Methods： Fifty female Wistar rats were randomly divided into 6 groups ：CPID model was induced in 4 groups,in addition to control group and false surgery group. The rats were given acupoint （Guanyuan and Zusanli） injection with astragalus parenteral solution and saline. The drug group was fed with Qianjinpian solutions. The histopathologic changes of uterus were observed and serum IL-2 and TNF-α levels detected. Results ： The rats in the model group showed chronic inflammatory changes,the animals in astragalus parenteral injection group and Qianjinpian group showed little histopathological changes. The serum TNF-α levels in the model group were significantly higher than those in the normal group and the astragalus parenteral injection group; while the IL-2 levels in model group were significantly lower than those of other 5 groups. The TNF-α and IL-2 levels in astragalus parenteral injection group were similar to those of normal group. Conclusion. The treatment of chronic pelvic inflammatory disease with acupoint injection of astragalus parenteral solution might be effective.