大鼠骨骼肌细胞的频域电生理特性研究

Electrophysiological Properties of Frequency Domain in Rats' Skeletal Muscle

目的:在100Hz～100MHz频率范围研究大鼠骨骼肌细胞的电生理特性,为进一步探讨骨骼肌疲劳或力竭的频域电特性研究奠定基础。方法:使用Agilent4294A阻抗分析仪测量大鼠腓肠肌组织的交流阻抗,通过细胞介电谱、Cole-Cole图、介电损失因子△ε″和介电损耗角正切△tgδ的频谱分析,建立了正常大鼠腓肠肌细胞的频域电生理特性参数(介电参数)。结果:(1)大鼠腓肠肌细胞介电响应具有频率依存性关系:介电常数ε随电场频率的增加而降低,电导率κ随频率的增加而上升;(2)大鼠腓肠肌细胞的频域介电参数(κL、κh、εL、εh、△ε″max、△tgδmax、fC1、fC2)在平行方向与垂直方向上存在差异;(3)交流电场对大鼠腓肠肌细胞的作用具有介电弛豫现象,表现为两个中心特征频率:第一特征频率fC1(∥)=fC1(⊥)=1.02kHz和第二特征频率fC2(∥)=59.31kHz,fC2(⊥)=380.04kHz。结论:利用本实验方法可获得骨骼肌细胞频域电生理指标,其中第一特征频率fC1和第二特征频率fC2是频域电特性的特色参数。

Objective To study the electrophysiological properties of rat skeletal muscle within the frequency from 100Hz to 100MHz. Methods The AC impedance of rat sural muscle tissues was measured using the Agilent 4294A impedance analyzer, then the electrophysiological properties of the normal rat sural muscle cells were established in the respect of cell dielectric spectroscopy, Cole - Cole plots, spectrum of loss factor and loss tangent. Results （ 1 ） The dielectric response of rat sural muscle cells was frequency - dependent, such as increase in frequency caused decrease in permittivity. At the same time the conductivity trended to arise ; （2） The dielectric parameters（κL, κh, εL,εh, △ε″max, Δtgδmax, fC1, fC2） cell fiber parallel to the applied electric field differed from that cell fiber perpendicular to the applied electric field; （3） The effect of alternating current electric field on rat sural muscle cells showed a dielectric dispersion phenomenon, representing two characteristic frequency： the 1st frequency（fC1（//） = fC1〈（⊥） = 1.02 kHz） and 2na frequency（fC2（//） = 59.31 kHz,fC2（⊥） = 380.04kHz） . Conclusion The method for studying the electrophysiological properties of rat skeletal muscle cells was established in the respect of the frequency domain, providing a possibility for investigating muscle fatigue in the future.