原发性肝细胞癌中p16、p15基因启动子区甲基化状态检测

Detection of methylation of p16 and p15 gene promoter in hepatocellular carcinoma

目的研究原发性肝细胞癌中p16、p15基因启动子区甲基化状态及其与原发性肝细胞癌发生发展的关系。方法用特异性甲基化PCR(MSP)法检测30例原发性肝细胞癌肿瘤组织和5例正常肝脏组织中p16、p15基因启动子区甲基化状态,并进行统计分析。结果30例原发性肝细胞癌肿瘤组织中p16和p15基因启动子区分别有53.3%(16/30,P<0.05)和46.7%(14/30,P>0.05)甲基化,5例正常组织中未发现甲基化。两基因甲基化与原发性肝细胞癌临床病理及HBsAg之间无明显相关性(P>0.05),两者在原发性肝细胞癌发生中有协同性(相关性和一致性)。结论p16、p15基因启动子区异常甲基化在原发性肝细胞癌中发生频率很高,可能对肝细胞癌发生发展起重要作用。

Objective To study the association of the presence p16 and p15 gene promoter methylation with hepatocellular carcinogenesis（HCC）. Methods The methylation status of p16 and p15 gene were evaluated in 30 HCC and 5 normal liver tissues using methylation specific polymerase chain reaction（MSP）. The data were statistically analyzed. Results Methylation of the p16 and p15 promoter were detected in HCC （53.3%, 16/30,P 〈 0. 05 ; 46.7% , 14/30, P 〉0. 05 ;respectively） ,while methylation were not found in normal liver tissues. A significant correlation was not found between methylation and clincopathological features, and HBsAg in HCC （ P 〉 0.05 ）. There was a cooperativity between p16 and p15 gene promoter methylation during hepatocellular carcinogenesis. Conclusion There is a high frequency of aberrant methylation of P16 and p15 gene promoter, and it may play a vital role in heptocarcinogenesis. It is of high value to provid a molecular marker to confirm the clinical diagnosis and gene therapy of HCC.