慢性乙型肝炎患者HBV DNA载量与肝组织病理的关系

Relationship between HBV DNA lood pathology of chronic hepatitis B

目的探讨慢性乙型肝炎(CHB)患者血清中HBVDNA含量与肝组织中HBV DNA含量的相关性以及血清HBV DNA水平与肝组织病理的关系。方法选取70名未经免疫或抗病毒治疗的CHB患者,用荧光PCR法检测CHB患者血清、肝组织中HBV DNA的含量,并与同期肝组织活检病理结果对比分析。结果肝组织中HBV DNA载量明显高于血清HBV DNA载量,但两者呈高度相关(r=0.882,P<0.05);肝组织病理分级与血清HBV DNA含量之间正相关关系(r=0.210,P<0.05)。结论肝组织HBV DNA水平较血清HBV DNA水平更能准确反映HBV DAN复制情况;随着血清HBV DNA浓度的增高,肝细胞炎症及肝细胞纤维化程度也随着加重。

Objective To explore the relationship between the contents of HBV DNA in serum and in liver tissue from chronic hepatitis B patients and the relationship between the level of HBV DNA in serum and hepatic pathology. Methods 70 patients in our hospital who had never accepted immune therapy or anti-virus treatment were chosen. Fluorescence quantitative PCR（FQ-PCR）was used to detect the quantity of HBV DNA not only in serum but also in liver tissue. Hepatic pathology was also used as control. Results The level of liver HBV DNA was much higher than that of serum HBV DNA（ t = 2. 214,P 〈 0. 05）. Liver HBV DNA level was positively linked to serum HBV DNA level（ r = 0. 882, P 〈 0. 05 ）. It was also correlated with hepatic pathology （ r = 0. 210, P 〈 0.05 ）. Conclusion Intrahepatic HBV DNA is a more valuable marker than serum HBV DNA for assesing HBV replication. As the inflammatory extent and fibrosis extent of hepatic tissue increas, the level of HBV DNA in serum and hepatic tissue displays middle and high distribution.