海水养殖花鲈鳃组织hepcidin-like抗菌肽基因克隆与序列分析

Cloning and sequence analysis of hepcidin-like antimicrobial peptide gene from gill of Japanese seabass(Lateolabrax japonicus)

本研究利用反转录-聚合酶链式反应(RT-PCR)和cDNA末端快速扩增技术(3′RACE)克隆出海水养殖花鲈(Lateolabrax japonicus)鳃组织hepcidin-like抗菌肽基因cDNA(523bp),包含完整的编码区、3′端非翻译区(3′UTR)和典型的多腺苷酸化信号(AATAAA).该基因编码86个氨基酸,由信号肽、优势域和成熟肽组成.与花鲈肝脏分离到hepc1及国外发表白鲈(Morone chrysops)hepcidin氨基酸序列进行比较,结果显示花鲈鳃hepcidin-like基因编码的氨基酸与它们在27个位点上存在着差异;该hepci-din-like基因可能为hepcidin的不同变体,属于hepcidin抗菌肽家族的新成员.

Using reverse transcription-polymerase chain reaction （RT-PCR） and rapid amplification of cDNA end （RACE）, a hepcidin-like AMP cDNA sequence （523bp） was isolated from gill of Japanese seabass （ Lateolabrax japonicus） following bacterial challenge, which consists of a complete coding sequence, a 3＇untranslated region and a canonical polyadenylation signal （AATAAA）. Putative peptide is 86 amino acids in length, and includes three domains： a signal peptide, a prodomain, and a mature peptide. Sequence analysis shows that the predicted hepcidin-like peptide differs from hepcl isolated from liver of Japanese seabass and the other hepcidin reported at 27 animo acid sites in white bass （Morone chrysops） , and it suggests that it may be another variant of the Japanese seabass hepcidin. The pioneer research indicates that the hepcidin from gill of the Japanese seabass is a new member of the hepcidin family.