3个海域沙筛贝遗传差异的DNA分子标记研究

DNA molecular markers of Mytilopsis sallei in three bays,for genetic difference

本文运用RAPD(random amplified polymorphic DNA)技术,对3个海域的沙筛贝进行了地理遗传差异分析.结果表明福建东山湾与厦门马銮湾沙筛贝的遗传距离为0.276 4,深圳湾与马銮湾沙筛贝的为0.306 7,深圳湾与东山湾沙筛贝的为0.330 5.这说明马銮湾与东山湾的沙筛贝种群遗传距离较近,而东山湾与深圳湾的沙筛贝种群遗传距离较大.对厦门马銮湾沙筛贝的18S rDNA进行了测序,在基因库上查得同科另外3个种的18S rDNA序列,构建了分子系统发育树,发现沙筛贝与Mytilopsisleucophaeata同源性比较高.

We use the RAPD （random amplified polymorphic DNA） technique to analyze the genetic differences among three populations of Mytilopsis sallei in three bays. The results showed that the genetic distance of Mytilopsis sallei between populations of Dongshan Bay and Maluan Bay was 0.276 4, while populaitons of Shenzhen Bay and Maluan Bay was 0. 306 7, and populations of Shenzhen Bay and Dongshan Bay was 0. 330 5. It means that the genetic distance of Mytilopsis sallei between Dongshan population and Maluan population was closer and the genetic distance of Mytilopsis sallei between Shenzhen population and Dongshan population was farther. The 18S rDNA of Mytilopsis sallei in Maluan Bay was sequenced and compared with other three species of Dreissenidae in gene bank and the molecular phylogenetic tree is constructed. The results showed that it is much homologous between Mytilopsis sallei and Mytilopsis leucophaeata among the four species.