职业性铅接触者红细胞嘧啶5＇－核苷酸酶、超氧化物歧化酶活性测定

The Activity Determination Activity of the Erythrocyte Pyrimidine 5'-nucleotidase and Superoxide Dismutase in Occupational Lead Exposed Workers

本文对１６名铅接触者、１４名铅中毒者和２０名非铅接触者进行了红细胞嘧啶５＇－核苷酸酶（Ｐ５Ｎ）和超氧化物歧化酶（ＳＯＤ）活性测定，并进行统计分析。结果表明铅可抑制红细胞Ｐ５Ｎ的活性，其抑制程度与铅毒效应密切相关：对照组（非接铅组）活性范围５．６～１４．２，平均１０．２μｍｏｌ尿嘧啶／ｈ／Ｈｂｇ／ｄｌ；接铅组活性范围为２．３～７．２，平均为４．４μｍｏｌ尿嘧啶／ｈ／Ｈｂｇ／ｄｌ，与非接铅组比较Ｐ＜０．００１；铅中毒组活性范围１．５～３．７，平均２．５μｍｏｌ尿嘧啶／ｈ／Ｈｂｇ／ｄｌ，与接铅组比较Ｐ＜０．００１。ＳＯＤ活性三组间无差别。

The present study determinated the activity of erythrocyte pyrimidine 5' -nucleotidase(P5N)and superoxide dismutase(SOD)in 16 lead-exposed workers,14 lead poisoning workers and 20 non lead exposed workers(the controls).The results showed that the P5N activity was significantly inhibited by lead,which was closely related to the toxic effect of lead:the activity in control group ranged from 5.6 to 14.2 μmoluridine/h/gHb/dl with a mean value of 10.2,l the leadexposed group ranged from 2.3 to7.2 μmol uridine/h/gHb/dl with a mean value of 4.4,significantly lower than that of the control group(P<0.001),the activity of P5N in lead poisoning group ranged from 1.5 to 3.7μmol uridine/gHb/dl with a mean value of 2.5,that was significantly lower than that lead-exposed group(P<0.001).The SOD activity did not show any obvious difference among three groups.It was suggested that the P5N activity might be one of the most liable indicators for evaluating the level of lead exposure.