卡托普利和缬沙坦对兔动脉粥样硬化心肌CRP表达的影响

The effects of captopril and valsartan on the expression of myocardial C-reactive protein in rabbit atherosclerotic myocardium

目的探讨卡托普利和缬沙坦从不同水平阻断血管紧张素Ⅱ(AngⅡ)后对心肌C反应蛋白(CRP)表达的影响。方法30只新西兰白兔随机分为四组(:A)高脂饮食组(、B)高脂饮食加卡托普利组(、C)高脂饮食加缬沙坦组(、D)对照组。喂饲10周后,放射免疫法测定血浆AngⅡ含量,取心肌和肝脏用免疫组化方法测定CRP的表达。结果A组心肌CRP表达较D组明显升高(P<0.005)。与A组相比,B组和C组心肌CRP表达显著降低(P<0.05,P<0.025),与D组相比,差异无统计学意义。剔除C组数据后,血浆AngⅡ水平与心肌局部CRP表达存在显著相关性(rs=0.804,P<0.0001)。肝脏CRP表达两两组间差异无统计学意义(P>0.05)。结论AngⅡ与心肌局部组织CRP的表达有显著的相关性;卡托普利和缬沙坦可降低心肌局部CRP的表达,差异无统计学意义。

Objective To observe the effects of captopril and valsartan on decreasing the expression of C-reactive protein （CRP） in rabbits atherosclerotic myocardium. Methods Thirty male New Zealand white rabbits were divided into four groups randomly ： （A） cholesterol feeded group, （ B ） cholesterol ＋ captopril-treated group, （C） cholesterol＋valsartan-treated group and （D） control group. After 10 weeks, plasma angiotensin Ⅱ （Ang Ⅱ ） level was evaluated by radioimmunoassay. The heart and liver were harvested for immunohistochemistry measure of CRP. Results Compared with control group, the expression of myocardial CRP in A group increased significantly （P〈0.005）. The levels of myocardial CRP in group B and group C was lower than that in group A （P〈0.05, P〈0.025 respectively）. There were no difference between group B and group C with control group. The expression of myocardial CRP in group B and group C were identical. After eliminating the data of group C, the expression of myocardial CRP was correlated positively with AngⅡ level of plasma. There was no difference in expression of hepatic CRP among the four groups. Conclusion Plasma Ang Ⅱ correlated positvely with the expression of myocardial CRP. Captopril and valsartan could reduce myocardial CRP. There was no difference in the effects on reducing myocardial CRP between captopril and valsartan. Ang Ⅱ influenced more on myocardial CRP than on hepatic CRP.