牛磺酸对肢体缺血再灌注致多器官损伤的预防作用研究

Prevention of distant multiple organ dysfunction caused by lower limbs ischemia/reperfusion with taurine

目的探讨牛磺酸在肢体缺血再灌注致多器官损伤的预防作用。方法（1）Wistar大鼠随机分三组：再灌注组，阻断双侧股动脉循环2h，再灌注5h；牛磺酸组，1～7d灌服20％牛磺酸3ml／只，后阻断双侧股动脉2h，再灌注5h；对照组，行假手术处理。（2）测定血白细胞总数、肝酶和心肌酶；观察红细胞SOD活性、血清谷胱甘肽（GSH）及肝、肺组织匀浆丙二醛（MDA）及器官的超微结构变化。结果再灌注组白细胞总数、心肌酶、肝酶较对照组显著降低（P〈0．05），其远隔器官超微病理示组织有弥漫性损伤。红细胞SOD及血清GSH较对照组显著下降，肝、肺组织匀浆MDA及肿瘤坏死因子含量显著增多（均P〈0．05）。而牛磺酸组病变明显改善。结论肢体缺血再灌注可激活中性粒细胞，释放氧自由基、肿瘤坏死因子，使机体处于全身炎症反应状态致远隔多器官损伤，牛磺酸可明显减轻多器官损伤。

Objective To investigate the prevention of distant multiple organ dysfunction caused by lower limb isehemia/reperfusion with taurine. Methods Wistar rats were randomly divided into 3 groups. （1）Reperfusion group： rats underwent 2-hour bilateral artery femoralis of hind limbs isehemia and followed by 5 hours reperfusion. （2）Taurine group：each rat was oraled 20 % taurine 3ml every day for 7 days and underwent 2hrs isehemia followed by 5 hrs reperfusion. （3） Control group： sham animals didn＇ t undergo isehemia. The count of leukocyte, myocardial and liver enzymes were measured. Ultrastructural pathological change of distant organs were observed. Superoxide dismutase（SOD） of erythrocyte, malondialdehyde（MDA） glutathione（GSH） and tumor necrosis factor（TNF） in tissue homogenization was assayed. Results The count of leukocyte was decreased significantly in reperfusion group, myocardial and liver enzymes were significantly increased compared with control group uhrastructural pathological change of extensive injury of distant multiple organs was occurred in perfusion group. SOD of erythrocyte and GSH in serum were decreased compared with control group, while MDA and tumor necrosis factor in tissue homogenization of liver and lung were significantly increased. The pathological changes of taurine group was obviously alleviate. Conclusion Limb ischemia/reperfusion can produce quantities of oxygen free radicals and TNF which were released by activated polymornuclear leukocytes. This made the body plunge into systemic inflammation response syndrome and induce distant multiple organ dysfunction. Taurine can prevent it obviously.