吸烟对老年脑梗死患者血小板GMP-140表达的影响

Platalet GMP-140 in cerebral infarction elders influenced by smoking

目的通过测定吸烟健康者和吸烟脑梗死患者全血血小板GMP-140水平,探讨吸烟致脑梗死和动脉粥样硬化形成的内在机制。方法用流式细胞仪测定32例吸烟脑梗死患者、30例不吸烟脑梗死患者、31例吸烟健康人和33例不吸烟健康人血小板GMP-140水平。结果GMP-140表达水平于脑梗死吸烟组(48.06±12.72)μg/mL、脑梗死不吸烟组(28.10±5.98)μg/mL、健康吸烟组(26.47±6.06)μg/mL明显高于健康不吸烟组(10.78±2.80)μg/mL(P<0.01);脑梗死吸烟组明显高于脑梗死不吸烟组(P<0.01);健康吸烟组明显高于健康不吸烟组(P<0.01)。重度吸烟组、中度吸烟组、轻度吸烟组GMP-140表达显著高于健康不吸烟组(P<0.01);重度吸烟组GMP-140表达明显高于轻度吸烟组(P<0.01);重度吸烟组GMP-140表达明显高于中度吸烟组(P<0.01)。GMP-140表达水平与吸烟指数呈正相关(r=0.556,P<0.01)。大梗死灶组和中梗死灶组GMP-140表达明显高于小梗死灶组(P<0.01);大梗死灶组GMP-140表达明显高于中梗死灶组(P<0.01)。结论吸烟使健康人和脑梗死患者GMP-140表达水平均升高。GMP-140表达水平升高与吸烟指数呈正相关。吸烟致GMP-140表达水平升高是吸烟致脑梗死的途径之一。

[Objective] To explore the role of smoking in atherosclerosis and cerebral infarction by testing GMP- 140 expressed on the surface of activated platalet. [Methods] Using Flow Cytometer（FCM） to test GMP-140 expressed on the surface of activated platalet in 32 cases of cerebral infarction smokers, 30 cases of cerebral infarction nonsmokers, 31 cases of healthy smokers and 33 cases of healthy nonsmokers. [Results] GMP-140 in the other three group of subjects that was smoking infarction group （48.06±12.72）μg/mL, nonsmoking infarction group （28.10± 5.98）μg/mL, smoking healthy group （26.47±6.06）μg/mL were all much higher than that of the nosmoking healthy group （10.78±2.80） μg/mL（P 〈0.01）. Cerebral infarction smokers and healthy smokers had higher level of GMP-140 respectivly compared with cerebral infarction nonsmokers（P 〈0.01 ）and healthy nonsmokers（P 〈0.01）. Smokers had more GMP-140 than nonsmokers （P 〈0.01）. Severe smoking cases had more GMP-140 than moderate ones （P 〈 0.01 ） and mild ones（P 〈0.01 ）. GMP-140 was positively correlated to smoking index （r =0.556, P 〈0.01）. GMP-140 increased significantly in big and moderate volume infarction group compared with the small one （P 〈0.01 ）; GMP- 140 increased significantly in big volume infarction group compared with the moderate one （P 〈0.01）. [Conclusions] Smoking elevate the level of GMO-140 in healthy smokers and cerebral infarction smokers. GMP-140 is positively correlated to smoking index and it is one of the reasons of cerebral infarction caused by cigarette smoking.