摘要
对小鼠肝微粒体CYP450酶系检测方法进行研究,应用差速离心法获取小鼠肝微粒体,采用B radford法检测肝微粒体蛋白浓度,用分光光度法检测CYP450酶系含量及活性,实验表明该方法具有便捷可靠,重复性好等特点。
To study the method of measuring cytochrome P450 （CYP450） in mouse liver microseme. Mouse liver microsome was obtained by different velocity centrifugation, the concentration of protein of liver microseme was measured by Bradford, and the activity of mice CYP450 was detected by spectrophotometer. The result showed that this method is easy and reliable, and has good reproducibility.
出处
《福建中医学院学报》
2006年第4期50-52,共3页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建省教育厅科研基金资助项目(JA04217)
