紫胶虫主要生产种的RAPD分子标记分析

Analysis of Relationships among the Main Commercial Species of Lac Insects Using Random Amplified Polymorphic DNA (RAPD)

用RAPD技术研究了紫胶虫7种主要生产种12个群体之间的亲缘关系，研究中每个群体使用了24个标本，共有288个个体用于研究。在试用的45种随机引物中，筛选出9种引物用于随机引物扩增，共得到121个清晰稳定的位点，其中118个为多态性位点，多态百分率为97．52％，每条引物检测到的位点数为11～16个，长度为150～3000bp。根据扩增结果，用UPGMA法对Nei＇s遗传距离进行聚类分析，构建分子系统树。7种紫胶虫聚类结果显示：紫胶虫各群体间的遗传距离为0．0443～0．7917，其中，种间的遗传平均距离为0．4430，种内的遗传平均距离为0．1707。根据聚类分析，讨论了紫胶虫主要生产种的亲缘关系。

The technique of random amplified polymorphic DNA （RAPD） was used to study the relationships of 12 populations from 7 species in the genus Kerria. A total of 288 samples were used in this study and 24 samples for each population. In 45 primers under predetermined optimal reaction conditions, 9 primers were informative and yielded a total of 121 clear and reproducible loci. The percentage of polymorphic loci （PPB） was 97.52%. The amplified DNA loci of individual primer were 11～16, and their molecular size was 150～3 000 bp. The dendrogram were constructed based on Nei＇s genetic distance by UPGMA method. The cluster results showed the genetic distance between inter-population were 0.044 3～0.791 7, in which the average genetic distance among species was 0.443 0 while under species was 0.170 7. By analysis of molecular dendrogram, the genetic relationships among production species were discussed.