转BAR基因水稻的抗性遗传、生理特性和农艺性状研究

Resistant inheritance,physiological and agricultural characters of BAR-transgenic rice.

为了明确BAR基因对水稻的影响,对转BAR基因的Bengal HU-10、Bengal HC-11、CypressPB-6、Cypress PB-13的抗性遗传、生理特性和农艺性状进行了研究,结果表明,它们对除草剂Liberty的抗性均受一对显性核基因控制,在这4个实验材料中,BAR基因在染色体上的整合位置互不等位,株高、生育期和每穗粒数等农艺性状也不相同.转BAR基因水稻与原亲本比较,叶片中叶绿素含量、光合速率和叶绿素荧光参数、GS酶活性等均未有明显差异,但用Liberty处理2 d后,上述生理性状变化明显,且在这4个转BAR基因材料之间也有显著差异,说明与BAR基因的插入位置有关.

In order to investigate the effect of BAR gene on rice, the characteristics of inheritance, physiology and agriculture of 4 BAR-transgenic rice （Bengal HU-10, Bengal HC-11, Cypress PB-6, Cypress PB-13） were studied. The results indicated that the resistance of this 4 BAR-transgenic rice to herbicide （Liberty） was controlled by a single dominant nuclear gene. The inserted locus of BAR gene and agricultural properties including plant height, growth period and grain number per panicle were different among the 4 BAR-transgenic rice. There were no evident differences in chlorophyll content, photosynthetic rate, parameters of chlorophyll fluorescence and glutarnine synthetase activity etc in leaves between BAR-transgenic rice and the original parents. However, the above physiological characters changed significantly and were significantly different between each BAR-transgenic rice in leaves 2 days later after spraying Liberty. The above results showed that the change of physiological characters was related to the inserted locus of BAR gene.