稻米透明度QTLs主效应、上位性效应和G×E互作效应检测

Analysis of QTLs with genetic main effect,additive×additive epistatic effect and G×E interaction effect of rice transparency.

利用珍汕97B/密阳46构建的RIL群体(ZM-RIL)及其相应分子遗传图谱,以海南和杭州两地试验的精米透光率(%)作为稻米透明度考察指标,应用检测QTL主效应、加×加上位性效应和G×E互作效应的遗传分析方法,对该性状两个环境下数据进行联合分析.共检测到5个控制该性状的主效应QTL,分别位于第2、6(2个)、8、10染色体上,总的遗传贡献率19.15%.其中,qTR2-2增加透明度的有效基因来源于母本;其余4个(qTR6-1、qTR6-2、qTR8-2、qTR10)则来自于父本.qTR6-1还与环境存在显著的GE互作效应.此外,还检测到2对控制稻米透明度的加性上位性互作基因,但它们均未与环境存在显著互作.

Recombinant inbred lines population derived from Zhenshan 97B/Miyang 46（ZS97B/MY46） and its genetic linkage map were used to map the QTLs controlling rice transparency in different environments, Hainan and Hangzhou. A total of five QTLs with genetic main effects were detected on chromosomes 2, 6 （two QTLs）, 8 and 10, which contributed to 19. 15% of the total phenotypic variations. Among them, the additive effects of qTR2-2 came from the female parent while the other four （qTR6-1, qTR6-2,qTRS-2,qTR10） came from the male parent. The QTL qTR6-1 was also detected to have significant G × E interaction effects. In addition, two pairs of QTLs with additive × additive epistasis were detected, but they had no G × E interaction effect.