摘要
用Westernblot和免疫荧光法检定狂犬病毒糖蛋白重组痘苗病毒(简称重组病毒),证实狂犬病毒糖蛋白在痘苗病毒中得以表达,分子量为64000道尔顿。实验表明,重组病毒增殖力比亲本株降低5-10倍,致细胞病变过程减缓,但增殖曲线和亲本株呈平行趋势,3天达到增殖高峰;重组病毒绝大部分在细胞内,仅不足1%释放到培养液中;在制备鸡胚细胞的同时接种重组病毒,3天收获,用超声波处理后,可提高病毒收获量,在最适条件下可达5xl0ltpFU/L。并探讨重组病毒及其亲本株的蚀斑形成单位(PFU)和血凝效价(HA)之间的相关关系。
Thefurtheridentificationofrecombinantvacciniavirus.expressingGproteinofrabiesvirusbyWestenblotandimmunofluorescentassaydemonstratedthatGproteinofrabiesvirushasbeenexpressedinvacciniavirus.withamolecu-larweightof64KD.Theexperimentalresultshowedthatthepropagatingabilityoftherecombinantvirusreducedby5-10times.TheprocessofCPEsloweddownfrom2to4days,Thenumbersoftherecombinantviruspresentingwithinthecellsandinthesupernatantwere99%* and1%* respectively.Weinoculatedtherecombinantvirusandpreparedchickembryofi-broblasts(CEF)simultaneously,andharestedthesample.oftheCEFinfectedwithrecotbinantvirus3daylaterandtreateditwithsupersonic,Bythismethod,amuchhigheryieldoftherecombinantvirushasbeenobtainedthanbyconventionalmethods.Themaximumyieldwasgreaterthan5xl0ttPFU/L.TheinterrelationshipbetweenPFUandHAassayofthere-combinantvirusandparentalviruswasalsodiscussedinthepresentpaper.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1996年第3期212-216,共5页
Chinese Journal of Experimental and Clinical Virology
关键词
重组痘苗病毒
狂犬病毒糖蛋白
病毒
VacciniarecombinantvirusRabiesvirusGproteinViralcytopathiceffect