苎麻纤维质酶降解生产燃料乙醇技术研究 I.外界因子对纤维质酶降解的影响

Technology of Producing Fuel Alcohol from Ramie Lignoellulose with Enzymes I.Environmental Factors' Effects on Ramie Lignocellulose Decomposed with Enzymes

在微生物预处理苎麻韧皮的基础上,进行了pH值、温度、浴比、无机盐、单糖种类等外界因子对苎麻纤维质酶降解的影响研究。结果表明,苎麻纤维质混合酶水解的适宜条件为:起始pH 5.0、温度50℃、浴比为1:15;纤维素酶水解最适条件为:起始pH 4.5、温度50℃,浴比为1:15;钙、镁离子对酶水解有较大的促进作用,铁离子和单糖对纤维质酶水解均有较强的抑制作用。

Some environmental factors affecting the decomposition of ramie cellulose including pH, temprature, ratio of water to material, inorganic ions and sugars were studied. The result showed that the most optimal condition for enzymatic decomposing is pHS. 0, 50℃, water to material ratiolS： 1 Ca^2＋ and Mg^2＋ can improve enzymatic decomposing greatly, Fe^3＋ and sugar, however, predominantly retard enzymatic decomposing.