魔角旋转核磁共振代谢组学方法对镧、铈急性生物效应的比较研究

Comparative Investigation on Acute Biological Effect of Lanthanum and Cerium by MAS^1H NMR-based Metabonomic Approach

利用高分辨魔角旋转核磁共振(MAS1H NMR)技术对腹腔注射不同剂量[2,10,50 mg/kg(体重)]的硝酸镧[La(NO3)3]和硝酸铈[Ce(NO3)3]的雄性W istar大鼠肝、肾组织的MAS1H NMR谱进行比较分析,研究了La(NO3)3和Ce(NO3)3的急性生物效应,并结合模式识别技术对不同剂量La(NO3)3和Ce(NO3)3的急性生物效应进行了分类.研究结果表明,La(NO3)3对大鼠的急性毒性主要表现为肝毒,Ce(NO3)3对大鼠肝、肾同时造成损伤.该方法可用于其它稀土及金属化合物的毒性预测和毒理学研究.

High-resolution magic-angle-spinning（MAS）^1 H NMR spectroscopic and pattern recognition （PR） based methods were applied to compare the different acute biochemical effects between La （NO3）3- and Ce（ NO3）3-treated rats. Male Wistar rats were treated with various doses（2, 10, 50 mg/kg body weight） of La（ NO3 ）3 and Ce（ NO3 ）3, and MAS l H NMR spectra of the intact liver and kidney tissues were analyzed by using principal components analysis to extract metabolic information. The target lesion of La （ NO3 ） 3 to liver and Ce （NO3）3 to both liver and kidney were found by MAS l H NMR-PR methods. This work illustrated that the combination of NMR and pattern recognition technique is a powerful method to study the biochemical effects induced by xenobiotics.