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快速老化模型小鼠血清蛋白质组学分析比较 被引量：2

Analysis and Comparison of Serum Proteomics of Senescence Accelerated Mice
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摘要 以6月及12月龄SAMP 8及同龄SAMR 1为研究对象,应用双向凝胶电泳法,分析比较了快速老化模型小鼠(Senescence accelerated m ice,SAM)的快速老化亚系SAMP 8及抗快速老化亚系SAMR 1血清蛋白表达的差异.与同龄SAMR 1比较,6月龄SAMP 8血清中有15个蛋白点表达显著上调,3个蛋白点表达显著下调,7个蛋白点只在SAMP 8中有表达;12月龄SAMP 8血清中有9个蛋白点表达显著上调,7个蛋白点表达显著下调,12个蛋白点只在SAMP 8中有表达.应用质谱进行肽质量指纹图谱分析和数据库检索共鉴定了19种蛋白质.其中6个蛋白只在6月龄SAMP 8中表达,4个蛋白只在12月龄SAMP 8中表达.此外,在6月龄及12月龄SAMP 8血清差异蛋白中,存在9个共同的差异蛋白,按照功能可分为4类:(1)免疫相关蛋白;(2)老化相关蛋白;(3)糖代谢及神经元凋亡相关蛋白;(4)其它蛋白.上述研究结果显示,SAMP 8和SAMR 1血清蛋白表达存在明显差异,其中一些差异蛋白可能是SAMP 8老化进程中相关病理生理变化的重要原因. To analyze and compare differential serum proteins of senescence accelerated mice： SAM-prone/8 （SAMP 8） and SAM-resistance/1 （SAMR 1 ）, the proteins of sera from 6 and 12 month-old SAMP 8 and age- matched SAMR 1 were se expressions in sera of sixparated, detected month-old SAMP and analyzed. Compared with the same age SAMR 1, 15 proteins 8 were up-regulated, 3 proteins expressions down-regulated siguifi- cantly and 7 proteins only expressed in SAMP 8 sera; 9 proteins expressions in sera of twelve-month-old SAMP 8 were up-regulated, 7 proteins expressions down-regulated significantly and 12 proteins only expressed in SAMP 8 sera. Using MALDI-TOF-MS, 19 proteins with significant changes were identified by peptide fingerprinting-map and the results searched in MASCOT database. The results showed that 6 proteins were only expressed in sera of six-month-old SAMP 8, 4 proteins only expressed in sera of twelve-month-old SAMP 8. In addition, there were 9 common proteins presenting both in sera of six and twelve-month-old SAMP 8. Our study showed that there were significant differences in serum protein expressions between SAMP 8 and SAMR 1, and some differential proteins may result in pathological changes during the course of aging.
作者 蒋宁 周文霞 张永祥 张学敏 王杰 刘炳玉
机构地区 军事医学科学院毒物药物研究所 国家生物医学分析中心
出处 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2006年第8期1462-1466,共5页 Chemical Journal of Chinese Universities
基金 国家自然科学基金(批准号:30200367) 国家'九七三'计划项目(批准号:2004CB518907 G1999054401)资助
关键词 快速老化模型小鼠 血清蛋白质组 双向凝胶电泳 Accelerated senescence mouse Serum proteomics Two dimensional gel electrophoresis
分类号 Q51 [生物学—生物化学]
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参考文献22

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共引文献6

同被引文献46

  • 1董雷,蒋宁,周文霞,张永祥,耿淼,张学敏,刘炳玉,王杰.快速老化模型小鼠海马蛋白质组学初步研究[J].高等学校化学学报,2007,28(2):274-277. 被引量：8
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高等学校化学学报
2006年 第8期

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