高效液相色谱法研究邻烯丙基苯酚在小麦植株中的向基传导和分布

Study on Transportation and Distribution of Fungicide o-AllylPhenol from the Leave to Root in Wheat Seedling by High Perofrmance Liquid Chromatography-Photodiode Array Detection

采用一种快速、灵敏的高效液相色谱-二极管阵列检测器法（HPLC-DAD）研究拟银杏杀菌剂邻烯丙基苯酚在小麦幼苗中的自顶向基传导作用,并定量分析其进入小麦植株后的具体分布.HPLC-DAD检测条件为：等度流动相65%乙腈/水（0.1%醋酸）,HYPERSIL BDS C18,5 μm, 250 mm×4.6 mm i.d色谱柱,检测波长280 nm.邻烯丙基苯酚的线性回归方程为Y=8.0266X＋5.019 （r=0.9999）,在2～500 mg/L浓度范围内线性关系良好.日内与日间精密度均≤12.44%;平均回收率为83.55%;最低检出限为1.5 ng, 最低定量限为2 mg/L.分析方法符合小麦体内药物含量测定的要求.经HPLC-DAD检测,确证了邻烯丙基苯酚具有自小麦叶部向基部传导的特性,施药4h后即可传至根部.给药处理72 h后药剂在小麦叶、茎及根部的积累水平相当,基本完成自顶向基传导过程.

A high-performance liquid ehromatography-photodiode array detector （HPLC-DAD） method was applied to determine transportation and distribution of the fungicide o-aUylphenol from the leave to root of wheat seedling. The samples were analyzed by HPLC-DAD on a reversed-phase C18 column and a mobile phase of acetonitrile/water （0. 1% acetic acid） （65：35, V/V） with ultraviolet detection at 280 nm. The equation of the calibration curves was Y = 8. 0266X ＋ 5. 019 （ r = 0. 9999 ）, and the linear ranges was 2 -500 mg/L for o-allylphenol. The intra- and inter-day precisions （RSD） were less than 12.4%. Mean recovery was 83.6%. The limit of detection and quantification were respectively 1.5 ng and 2.00 mg/L. The validated method was applicable determination of o-allylphenol from wheat extracts. The study indicated that o-allylphenol had rapid transportation and distribution function in wheat seedling. The fungicide transported already to the root of wheat at 4 h after treatment. The accumulative levels were similar in whole seedling including leaves, stem and root and completed basically the process transporting from the top down at 72 h.