胶束增敏荧光法测定山楂中总黄酮含量

Determination of Total Flavanone Quantity in Hawthorn by Micellar Enhanced Fluorimetry

以金丝桃苷为对照品用荧光法测定山楂中总黄酮量。实验表明:乙醇胺与金丝桃苷作用可产生强的荧光,加入CTMAB后,荧光强度进一步增强。金丝桃苷在4×10-7—1.5×10-5mol/L浓度范围内与其荧光强度呈良好的线性关系,其线性回归方程为:y=3.085×107x+122.56;检出限为2.4×10-7mol/L。本方法用于山楂中总黄酮量的测定,其回收率为95.8%—100.0%,RSD为1.64%。

The fluorimetry was applied to determine the total flavanone quantity of hawthorn and hyperin was used as the comparison. The experimental conditions were optimized as follows.absolute alcohol was used as solvent,the amounts of pure ethanolamine and surface active agent CTMAB（1.2 × 10^-3mol/L） was 0. 5mL. and 0. 6mL, respectively, the solution was determined under the room temperature. At maximum excitation wavelength of 457nm and emission wavelength of 531nm, the fluorescence intensity was measured. The detective limits is 2.4 × 10^-7mol/L in the linear range of 4 × 10^-7-1. 5 × 10^-5mol/L,RSD of the method is 1. 64%. The method is simple,rapid,and accurate with a potential in analytical applications.