转基因鱼腥藻中hTNF-α基因表达与光合作用间的相关性研究

Studies on Relationship Between the Expression of hTNF-α Gene and Photosynthesis in Anabaena sp. IB02

分析了培养光强对转基因鱼腥藻生长和hTNF-α基因表达的影响,以及转基因鱼腥藻IB02的光合放氧活性、光系统Ⅰ及光系统Ⅱ活性。发现光强对转基因鱼腥藻IB02的生长和hTNF-α基因表达都有促进;hTNF-α基因在鱼腥藻中的表达率与真正光合、光系统Ⅰ和光系统Ⅱ活性存在一定的联系。hTNF-α基因表达同时对宿主的光合放氧特性也产生了显著的影响,与正对照相比转基因藻光呼吸速率增强68%,饱和点降低66%,说明转基因鱼腥藻的代谢负荷增加,并在低光强下生长比野生型快。

The effects of illumination on growth of Anabaena sp. IB02 and hTNF-α expression were studied. Photosynthetic activity, PS Ⅰ and PS Ⅱ activity of Anabaena sp. IB02 were assayed. Illumination enhanced the growth of Anabaena sp. IB02 and hTNF-α expression. Some relations were observed between hTNF-α expression and ture photosynthesis activity, PS Ⅰ , PS Ⅱ activity of Anabaena sp. IB02. Significant differences of the photosynthetic activity of host were detected simultaneously when hTNF-α expressed： the respiration rate increased（ - 68 % ）, the light saturation point descended（ ＋ 66% ）, all these suggested that the metabolic charge of host were increased and grow faster than wild type under low illumination.