子宫颈上皮细胞癌变中树突状细胞分布、TNF-α、c-myc的表达及其临床意义

The expression and clinic implication of dendritic cells,TNF-α and c-myc in the carcinogenesis of uterine cervical epithelial tissue

目的:探讨子宫颈上皮正常对照组织、非典型增生组织及子宫颈癌组织中树突状细胞(DC)的形态分布改变及肿瘤坏死因子-α(TNF-α)、c-myc表达的意义,以及它们与肿瘤临床病理参数间的关系。方法:应用免疫组化S-P法检测子宫颈上皮正常对照组织、非典型增生组织各15例及40例子宫颈癌组中DC的形态分布特征及TNF-αc、-myc的表达。结果:子宫颈上皮非典型增生组及子宫颈癌组中DC数量显著高于正常对照组(P<0.001);子宫颈上皮非典型增生组中DC呈树枝状,有较多分支,子宫颈癌组中DC散在分布,位于癌巢中的DC多呈椭圆形或圆形,而位于间质中的DC呈树枝状,有较多分支;TNF-αc、-myc的表达在子宫颈癌组、非典型增生组及正常对照组间差异具有统计学意义(P<0.001)。子宫颈癌中DC的表达与患者的年龄、病理分级、临床分期及肿瘤直径有关(P<0.05);TNF-α的表达与患者的临床分期及肿瘤直径有关,而c-myc的表达仅与患者的临床分期有关(P<0.05)。结论:DC、TNF-α、c-myc在子宫颈上皮癌变过程及发展中起重要作用,并且与肿瘤临床病理有密切关系。

Objective： To study the expression and implication of dendritic cells （DC）,TNF-αand c-myc in uterine cervical tissue. Methods： Fifteen eases of normal uterine cervix, fifteen cases of cervical epithelial dysplasia and forty cases with cervical cancer were identified the expression of DC, TNF-α and c-myc by immunehistochemical method （S-P）. Results： The number of DC in cervical epithelial dysplasia and cervical cancer was higher than that in normal uterine cervix （P〈0.001）. The expression of TNF-α, c-myc cervical cancer was higher than that in normal uterine cervix and in cervical epithelial dysplasia （P〈0.001）. In cervical cancer, the expression of DC was positively related to the age, degree of differentiation, clinical stages and tumor diameter, TNF-α was positively related to clinic stages and tumor diameter,and c-myc was positively related to clinical stages. Conclusion： DC, TNF-α, c-myc may play important role in the pathogenesis and development of cervical squmous carcinoma, and they were tightly correlated with clinicopathologic factors, especially DC may act as a predictor of prognosis of cervical cancer.